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Literature summary extracted from

  • Giorio, G.; Yildirim, A.; Stigliani, A.L.; DAmbrosio, C.
    Elevation of lutein content in tomato: a biochemical tug-of-war between lycopene cyclases (2013), Metab. Eng., 20, 167-176.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.18 gene Lcy-e, expression in tomato fruits under control of the 35S CaMV promoter in cotyledons via Agrobacterium tumefaciens-mediated transformation, the transgenic plants show increased lutein levels, quantitative real-time quantitative PCR expression analysis Solanum lycopersicum
5.5.1.19 gene Lcy-b, expression in tomato fruite under control of the 35S CaMV promoter in cotyledons via Agrobacterium tumefaciens-mediated transformation, the transgenic plants show increased lutein levels, quantitative real-time quantitative PCR expression analysis Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.18 additional information for production of lutein in tomato fruits, it is required to overcome the enzyme downregulation before fruit ripening exhibited through a tightly regulated physiological mechanism in tomato fruits by overexpression of lycopene epsilon- and beta-cyclases in leaves and ripe fruits, carotene profile and contents, overview Solanum lycopersicum
5.5.1.19 additional information for production of lutein in tomato fruits, it is required to overcome the enzyme downregulation before fruit ripening exhibited through a tightly regulated physiological mechanism in tomato fruits by overexpression of lycopene epsilon- and beta-cyclases in leaves and ripe fruits, carotene profile and contents, overview Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.18 lycopene Solanum lycopersicum
-
delta-carotene
-
?
5.5.1.19 lycopene Solanum lycopersicum
-
gamma-carotene
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.18 Solanum lycopersicum
-
cv. Red Setter, gene Lcy-e
-
5.5.1.19 Solanum lycopersicum
-
cv. Red Setter, gene Lcy-b
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.18 fruit
-
Solanum lycopersicum
-
5.5.1.19 fruit
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.18 lycopene
-
Solanum lycopersicum delta-carotene
-
?
5.5.1.19 lycopene
-
Solanum lycopersicum gamma-carotene
-
?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.18 LCY-E
-
Solanum lycopersicum
5.5.1.18 lycopene epsilon cyclase
-
Solanum lycopersicum
5.5.1.19 LCY-B
-
Solanum lycopersicum
5.5.1.19 lycopene beta cyclase
-
Solanum lycopersicum

Expression

EC Number Organism Comment Expression
5.5.1.18 Solanum lycopersicum the enzyme is downregulated before fruit ripening through a tightly regulated physiological mechanism down
5.5.1.19 Solanum lycopersicum the enzyme is downregulated before fruit ripening through a tightly regulated physiological mechanism down

General Information

EC Number General Information Comment Organism
5.5.1.18 metabolism the enzyme is downregulated before fruit ripening through a tightly regulated physiological mechanism Solanum lycopersicum
5.5.1.19 metabolism the enzyme is downregulated before fruit ripening through a tightly regulated physiological mechanism Solanum lycopersicum