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Literature summary extracted from

  • Blumhoff, M.L.; Steiger, M.G.; Mattanovich, D.; Sauer, M.
    Targeting enzymes to the right compartment: metabolic engineering for itaconic acid production by Aspergillus niger (2013), Metab. Eng., 19, 26-32.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.6 gene cadA, the cadA gene of is located in a gene cluster together with two transporters, one a putative mitochondrial carrier, the other a putative plasma membrane carrier, cloning in Escherichia coli strain TOP10, heterologous mitochondrial and cytosolic expression of the enzyme in Aspergillus niger strain ATCC 1015, the mitochondrial expression doubles the enzyme productivity compared to the cytosolic expression. Co-expression of a cytosolic or a mitochondrial aconitase with cytosolic CadA leads to a significant increase of itaconic acid production compared to cytsolic CadA expression alone. The rate-limiting step for itaconic acid production under these conditions appears to be both the provision of cis-aconitate as a substrate for the CadA as well as the CAD activity itself Aspergillus terreus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.6 cytosol cytosolic isozyme Aspergillus terreus 5829
-
4.1.1.6 mitochondrion mitochondrial isozyme Aspergillus terreus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.6 cis-aconitate Aspergillus terreus
-
itaconate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.6 Aspergillus terreus
-
gene cadA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.6 cis-aconitate
-
Aspergillus terreus itaconate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.6 CAD
-
Aspergillus terreus
4.1.1.6 cis-aconitate decarboxylase
-
Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.6 37
-
assay at Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.6 80
-
recombinant enzyme in cell free extract, 20 min, inactivation Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.6 6.2
-
assay at Aspergillus terreus