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Literature summary extracted from

  • Coquille, S.; Roux, C.; Mehta, A.; Begley, T.P.; Fitzpatrick, T.B.; Thore, S.
    High-resolution crystal structure of the eukaryotic HMP-P synthase (THIC) from Arabidopsis thaliana (2013), J. Struct. Biol., 184, 438-444.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.17 gene thiC, recombinant expression of His-tagged N-terminally truncated mutant DELTAN71-AtTHIC in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.99.17 purified recombinant His-tagged DELTAN71-AtTHIC mutant, by a proteolysis assay (especially alpha-chymotrypsin) coupled with the sitting drop vapor diffusion technique at 18°C, using 0.01 M cobalt (II) chloride hexahydrate, 0.1 M sodium acetate trihydrate, pH 4.6, 1 M 1,6-hexanediol, X-ray diffraction structure determination and analysis at 1.6 A resolution Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.17 additional information generation of an N-terminally truncated version of Arabidopsis thaliana THIC, lacking the first 71 amino acids, a chloroplastidial targeting peptide. The truncated AtTHIC is functionally active Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.99.17 chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.17 Co2+ a metal ion with octahedral coordination (two strictly conserved histidine residues (H426 and H490) and four water molecules) at the same location as a zinc ion in the bacterial enzyme, and a metal ion with multiple coordinated water molecules in the close vicinity of the substrate binding sites, binding structures, overview Arabidopsis thaliana
4.1.99.17 Fe2+ the enzyme contains a [4Fe-4S] cluster Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.17 65451
-
 2 * 65451, recombinant His-tagged DELTAN71-AtTHIC mutant, mass spectrometry and crystal structure Arabidopsis thaliana
4.1.99.17 130000
-
 recombinant His-tagged DELTAN71-AtTHIC mutant, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Arabidopsis thaliana
-
 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.17 Arabidopsis thaliana O82392 gene thiC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.17 recombinant His-tagged N-terminally truncated mutant DELTAN71-AtTHIC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Arabidopsis thaliana 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme uses an iron-sulfur cluster as well as a 5'-deoxyadenosyl radical as cofactors to rearrange the 5-amino-imidazole ribonucleotide (AIR) substrate to the pyrimidine ring Arabidopsis thaliana 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.17 dimer 2 * 65451, recombinant His-tagged DELTAN71-AtTHIC mutant, mass spectrometry and crystal structure Arabidopsis thaliana
4.1.99.17 More enzyme three-dimensional structure analysis, overview Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.1.99.17 HMP-P synthase
-
 Arabidopsis thaliana
4.1.99.17 thiC
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.17 S-adenosyl-L-methionine a S-adenosyl-L-methionine radical enzyme Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.1.99.17 metabolism the enzyme is important in thiamine diphosphate, vitamin B1, biosynthesis, an essential cofactor for key cellular metabolic enzymes in all forms of life Arabidopsis thaliana
4.1.99.17 additional information enzyme three-dimensional structure analysis and comparison, overview Arabidopsis thaliana