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Literature summary extracted from

  • Gaid, M.M.; Scharnhop, H.; Ramadan, H.; Beuerle, T.; Beerhues, L.
    4-Coumarate:CoA ligase family members from elicitor-treated Sorbus aucuparia cell cultures (2011), J. Plant Physiol., 168, 944-951.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expression of GST-tagged isozyme Sa4CL1 in Escherichia coli Sorbus aucuparia
6.2.1.12 expression of His6-tagged isozyme Sa4CL2 in Escherichia coli Sorbus aucuparia
6.2.1.12 expression of His6-tagged isozyme Sa4CL3 in Escherichia coli Sorbus aucuparia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 additional information
-
 additional information steady-state Michaelis–Menten kinetics Sorbus aucuparia
6.2.1.12 0.0009
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.0009
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.0025
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.0054
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.0068
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.0073
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.009
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.0125
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.021
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.023
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.033
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.05
-
 ferulate pH 7.5, 22°C Sorbus aucuparia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.12 chloroplast isozyme Sa4CL3 contains a 49-amino acid N-terminal extension, which includes a chloroplast sorting signal Sorbus aucuparia 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Ca2+ can substitute Mg2+ Sorbus aucuparia
6.2.1.12 Mg2+ required Sorbus aucuparia
6.2.1.12 Mn2+ can substitute Mg2+ Sorbus aucuparia
6.2.1.12 additional information strict dependence of Sa4CLs on a divalent cation, no activity with Cu2+ Sorbus aucuparia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 59500
-
 x * 59500, about, isozyme Sa4CL1, sequence calculation Sorbus aucuparia
6.2.1.12 60100
-
 x * 60100, about, isozyme Sa4CL2, sequence calculation Sorbus aucuparia
6.2.1.12 66200
-
 x * 66200, about, isozyme Sa4CL3, sequence calculation Sorbus aucuparia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Sorbus aucuparia
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA Sorbus aucuparia
-
 AMP + diphosphate + cinnamoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Sorbus aucuparia D5LLN7 isozyme Sa4CL1; isozyme Sa4CL1
-
6.2.1.12 Sorbus aucuparia D5LNB5 isozyme Sa4CL2; isozyme Sa4CL2
-
6.2.1.12 Sorbus aucuparia D5LNB6 isozyme Sa4CL3; isozyme Sa4CL3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 cell culture
-
 Sorbus aucuparia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Sorbus aucuparia AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA preferred substrate Sorbus aucuparia AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Sorbus aucuparia AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Sorbus aucuparia AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Sorbus aucuparia AMP + diphosphate + ferulyl-CoA
-
 ?
6.2.1.12 additional information no activity with benzoate and sinapate Sorbus aucuparia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 ? x * 59500, about, isozyme Sa4CL1, sequence calculation Sorbus aucuparia
6.2.1.12 ? x * 60100, about, isozyme Sa4CL2, sequence calculation Sorbus aucuparia
6.2.1.12 ? x * 66200, about, isozyme Sa4CL3, sequence calculation Sorbus aucuparia

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate:CoA ligase
-
 Sorbus aucuparia
6.2.1.12 Sa4CL1
-
 Sorbus aucuparia
6.2.1.12 Sa4CL2
-
 Sorbus aucuparia
6.2.1.12 Sa4CL3
-
 Sorbus aucuparia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 30
-
-
 Sorbus aucuparia
6.2.1.12 37
-
-
 Sorbus aucuparia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 0.26
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.3
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.34
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.36
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.37
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.38
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.49
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.58
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.78
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.8
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 0.96
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 1
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 6.5
-
-
 Sorbus aucuparia
6.2.1.12 7.5
-
-
 Sorbus aucuparia

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Sorbus aucuparia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.12 Sorbus aucuparia sequence calculation
-
 5.5
6.2.1.12 Sorbus aucuparia sequence calculation
-
 5.6

General Information

EC Number General Information Comment Organism
6.2.1.12 evolution phylogenetically, isozymes Sa4CL1 and Sa4CL2 belong to the class I cluster and Sa4CL3 groups in the class II cluster Sorbus aucuparia
6.2.1.12 metabolism the enzyme is involved in the flavonoid biosynthesis from cinnamic acid, it is the last enzyme of the general phenylpropanoid pathway, overview Sorbus aucuparia
6.2.1.12 physiological function Sorbus aucuparia cell cultures accumulate biphenyl and dibenzofuran phytoalexins from the starter substrate benzoyl-CoA in response to elicitor treatment involving cinnamate:CoA ligase and benzoate:CoA ligase, but not 4-coumarate:CoA ligase Sorbus aucuparia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.12 10
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 10
-
 cinnamate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 60
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 90
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 120
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 120
-
 caffeate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 130
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 230
-
 4-coumarate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 360
-
 ferulate pH 7.5, 22°C Sorbus aucuparia
6.2.1.12 410
-
 caffeate pH 7.5, 22°C Sorbus aucuparia