EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.10 | 0.38 | - |
(R)-mandelonitrile | native protein, pH 5.5, 25°C | Prunus turcomanica | |
4.1.2.10 | 0.95 | - |
(R)-mandelonitrile | enzyme immobilized onto Eupergit C250 L, pH 5.5, 25°C | Prunus turcomanica | |
4.1.2.10 | 1 | 4 | benzaldehyde | native protein, pH 5.5, 25°C | Prunus turcomanica | |
4.1.2.10 | 1.3 | - |
(R)-mandelonitrile | enzyme immobilized onto Eupergit CM, pH 5.5, 25°C | Prunus turcomanica | |
4.1.2.10 | 38 | - |
benzaldehyde | enzyme immobilized onto Eupergit C250 L, pH 5.5, 25°C | Prunus turcomanica | |
4.1.2.10 | 41 | - |
benzaldehyde | enzyme immobilized onto Eupergit CM, pH 5.5, 25°C | Prunus turcomanica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 25000 | - |
4 * 25000, SDS-PAGE | Prunus turcomanica |
4.1.2.10 | 100000 | - |
gel filtration | Prunus turcomanica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.10 | Prunus turcomanica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.10 | - |
Prunus turcomanica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.2.10 | seed | - |
Prunus turcomanica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.10 | (R)-mandelonitrile | - |
Prunus turcomanica | cyanide + benzaldehyde | - |
? | |
4.1.2.10 | cyanide + 2-chlorobenzaldehyde | - |
Prunus turcomanica | (R)-2-chloromandelonitrile | - |
? | |
4.1.2.10 | cyanide + 3,4-dihydroxybenzaldehyde | - |
Prunus turcomanica | (R)-3,4-dihydroxymandelonitrile | - |
? | |
4.1.2.10 | cyanide + 4-phenylbutan-2-one | - |
Prunus turcomanica | 2-hydroxy-4-phenylbutyronitrile | - |
? | |
4.1.2.10 | cyanide + benzaldehyde | - |
Prunus turcomanica | (R)-mandelonitrile | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.10 | tetramer | 4 * 25000, SDS-PAGE | Prunus turcomanica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 5 | - |
carboligation reaction | Prunus turcomanica |
4.1.2.10 | 25 | - |
mandelonitrile cleavage reaction | Prunus turcomanica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.10 | 4 | - |
carboligation reaction | Prunus turcomanica |
4.1.2.10 | 5.5 | - |
mandelonitrile cleavage reaction, enzyme immobilized onto Eupergit CM and Eupergit C 250 L supports | Prunus turcomanica |
4.1.2.10 | 6 | - |
mandelonitrile cleavage reaction, native enzyme | Prunus turcomanica |