BRENDA - Enzyme Database

The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA

Grant, T.D.; Luft, J.R.; Wolfley, J.R.; Snell, M.E.; Tsuruta, H.; Corretore, S.; Quartley, E.; Phizicky, E.M.; Grayhack, E.J.; Snell, E.H.; J. Mol. Biol. 425, 2480-2493 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.18
expressed in Saccharomyces cerevisiae strain BCY123
Saccharomyces cerevisiae
Crystallization (Commentary)
EC Number
Crystallization
Organism
6.1.1.18
microbatch-under-oil method, using 50 mM NH4Br, 50 mM KC2H3O2, 100 mM HEPES (pH 7.5), and 20% (w/v) polyethylene glycol 20000
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.18
89100
-
x * 89100, small-angle X-ray scattering
Saccharomyces cerevisiae
6.1.1.18
93100
-
x * 93100, calculated from amino acid sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanobacterium thermoautotrophicus
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
Saccharomyces cerevisiae
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
Saccharomyces cerevisiae ATCC 204508
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
Thermotoga maritima
-
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Methanobacterium thermoautotrophicus
O26157
-
-
6.1.1.18
Saccharomyces cerevisiae
P13188
-
-
6.1.1.18
Saccharomyces cerevisiae ATCC 204508
P13188
-
-
6.3.5.7
Thermotoga maritima
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.18
IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
728154
Methanobacterium thermoautotrophicus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
-
728154
Saccharomyces cerevisiae
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
-
728154
Saccharomyces cerevisiae ATCC 204508
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
-
728154
Thermotoga maritima
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.18
?
x * 89100, small-angle X-ray scattering; x * 93100, calculated from amino acid sequence
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Methanobacterium thermoautotrophicus
6.1.1.18
ATP
-
Saccharomyces cerevisiae
6.3.5.7
ATP
-
Thermotoga maritima
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.18
expressed in Saccharomyces cerevisiae strain BCY123
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Methanobacterium thermoautotrophicus
6.1.1.18
ATP
-
Saccharomyces cerevisiae
6.3.5.7
ATP
-
Thermotoga maritima
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
6.1.1.18
microbatch-under-oil method, using 50 mM NH4Br, 50 mM KC2H3O2, 100 mM HEPES (pH 7.5), and 20% (w/v) polyethylene glycol 20000
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.18
89100
-
x * 89100, small-angle X-ray scattering
Saccharomyces cerevisiae
6.1.1.18
93100
-
x * 93100, calculated from amino acid sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Methanobacterium thermoautotrophicus
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
Saccharomyces cerevisiae
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
Saccharomyces cerevisiae ATCC 204508
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
Thermotoga maritima
-
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.18
IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
728154
Methanobacterium thermoautotrophicus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
-
728154
Saccharomyces cerevisiae
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
-
?
6.1.1.18
ATP + L-glutamine + tRNAGln
-
728154
Saccharomyces cerevisiae ATCC 204508
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine + H2O
-
728154
Thermotoga maritima
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.18
?
x * 89100, small-angle X-ray scattering; x * 93100, calculated from amino acid sequence
Saccharomyces cerevisiae