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Literature summary extracted from

  • Grant, T.D.; Luft, J.R.; Wolfley, J.R.; Snell, M.E.; Tsuruta, H.; Corretore, S.; Quartley, E.; Phizicky, E.M.; Grayhack, E.J.; Snell, E.H.
    The structure of yeast glutaminyl-tRNA synthetase and modeling of its interaction with tRNA (2013), J. Mol. Biol., 425, 2480-2493.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.18 expressed in Saccharomyces cerevisiae strain BCY123 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.18 microbatch-under-oil method, using 50 mM NH4Br, 50 mM KC2H3O2, 100 mM HEPES (pH 7.5), and 20% (w/v) polyethylene glycol 20000 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.18 89100
-
 x * 89100, small-angle X-ray scattering Saccharomyces cerevisiae
6.1.1.18 93100
-
 x * 93100, calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu Methanobacterium thermoautotrophicus
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln Saccharomyces cerevisiae
-
 AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln Saccharomyces cerevisiae ATCC 204508
-
 AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine + H2O Thermotoga maritima
-
 ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Methanobacterium thermoautotrophicus O26157
-
-
6.1.1.18 Saccharomyces cerevisiae P13188
-
-
6.1.1.18 Saccharomyces cerevisiae ATCC 204508 P13188
-
-
6.3.5.7 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.18 IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Methanobacterium thermoautotrophicus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Saccharomyces cerevisiae AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Saccharomyces cerevisiae ATCC 204508 AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine + H2O
-
 Thermotoga maritima ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.18 ? x * 89100, small-angle X-ray scattering Saccharomyces cerevisiae
6.1.1.18 ? x * 93100, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 GluRS
-
 Methanobacterium thermoautotrophicus
6.1.1.17 Glutamyl-tRNA synthetase
-
 Methanobacterium thermoautotrophicus
6.1.1.18 Gln4
-
 Saccharomyces cerevisiae
6.1.1.18 GlnRS
-
 Saccharomyces cerevisiae
6.1.1.18 Glutaminyl-tRNA synthetase
-
 Saccharomyces cerevisiae
6.3.5.7 GatB
-
 Thermotoga maritima
6.3.5.7 GatCAB amidotransferase
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.17 ATP
-
 Methanobacterium thermoautotrophicus
6.1.1.18 ATP
-
 Saccharomyces cerevisiae
6.3.5.7 ATP
-
 Thermotoga maritima