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Literature summary extracted from

  • Kadziola, A.; Jepsen, C.H.; Johansson, E.; McGuire, J.; Larsen, S.; Hove-Jensen, B.
    Novel class III phosphoribosyl diphosphate synthase: structure and properties of the tetrameric, phosphate-activated, non-allosterically inhibited enzyme from Methanocaldococcus jannaschii (2005), J. Mol. Biol., 354, 815-828.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate activates, maximal activity at 190 mM phosphate Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expressed in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.1 hanging-drop vapour-diffusion technique Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP a single ADP binding site, the active site, is present per subunit Methanocaldococcus jannaschii
2.7.6.1 alpha,beta-methylene ATP
-
 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 2.6
-
 ATP pH and temperature not specified in the publication Methanocaldococcus jannaschii
2.7.6.1 2.8
-
 D-ribose 5-phosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ the activity of the enzyme depends on the presence of divalent cations, of which Mg2+ is the most effective. Mn2+ is also accepted, but the activity is less than 15% of that obtained with Mg2+, when assayed at pH 9.5 Methanocaldococcus jannaschii
2.7.6.1 Mn2+ the activity of the enzyme depends on the presence of divalent cations, of which Mg2+ is the most effective. Mn2+ is also accepted, but the activity is less than 15% of that obtained with Mg2+, when assayed at pH 9.5 Methanocaldococcus jannaschii
2.7.6.1 additional information the enzyme is essentially inactive in the presence of Cu2+, Ca2+, Co2+, Ni2+ or Fe2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 32229
-
 4 * 32229, calculated from sequence Methanocaldococcus jannaschii
2.7.6.1 147000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Methanocaldococcus jannaschii Q58761
-
-
2.7.6.1 Methanocaldococcus jannaschii DSM 2661 Q58761
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1
-
 Methanocaldococcus jannaschii

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 4°C or -20°C in 25 mM Tris–HCl (pH 7.6), 50% (v/v) glycerol, purified enzyme is stable for at least 90 days when stored at Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 ATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii DSM 2661 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 dATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 dATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii DSM 2661 dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 additional information the enzyme is essentially unable to use GTP (0.4% of the activity obtained with ATP), UTP (0.5%) or CTP (3%) as phosphoryl donor Methanocaldococcus jannaschii ?
-
 ?
2.7.6.1 additional information the enzyme is essentially unable to use GTP (0.4% of the activity obtained with ATP), UTP (0.5%) or CTP (3%) as phosphoryl donor Methanocaldococcus jannaschii DSM 2661 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 tetramer 4 * 32229, calculated from sequence Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 phosphoribosyl diphosphate synthase
-
 Methanocaldococcus jannaschii
2.7.6.1 PRPP synthase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 85
-
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.6.1 72 95 70% of maximal activity at 72°C and 95°C Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.1 85
-
 at pH 9.0, half-life: 8 min Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 9.5
-
-
 Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.1 10
-
 the activity profile declines steeply above pH 10. At least in part, this reduction in activity at high pH values may be caused by the formation of a magnesium phosphate precipitate, and consequently cause Mg2+ depletion. At lower pH the activity increases more or less linearly from pH 6.0 to pH 9.5 Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.057
-
 ADP pH and temperature not specified in the publication Methanocaldococcus jannaschii
2.7.6.1 0.85
-
 alpha,beta-methylene ATP pH and temperature not specified in the publication Methanocaldococcus jannaschii