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Literature summary extracted from

  • Cho, M.H.; Shin, Y.W.; Chun, J.H.; Hong, K.J.; Na, B.K.; Rhie, G.E.; Seong, B.L.; Yoo, C.K.
    Expression and characterization of an iron-containing superoxide dismutase from Burkholderia pseudomallei (2012), J. Microbiol., 50, 1029-1033.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene sodB, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Escherichia coli strain BL21(DE3) Burkholderia pseudomallei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 hydrogen peroxide
-
 Burkholderia pseudomallei
1.15.1.1 additional information no inhibition by sodium azide or potassium cyanide Burkholderia pseudomallei
1.15.1.1 paraquat
-
 Burkholderia pseudomallei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ a Fe-SOD Burkholderia pseudomallei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 25000
-
 x * 25000, recombinant His-tagged enzyme, SDS-PAGE Burkholderia pseudomallei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ Burkholderia pseudomallei
-
 O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+ Burkholderia pseudomallei 1106a
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Burkholderia pseudomallei A3NS93 isolated from a melioidosis patient in Korea
-
1.15.1.1 Burkholderia pseudomallei 1106a A3NS93 isolated from a melioidosis patient in Korea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Burkholderia pseudomallei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+
-
 Burkholderia pseudomallei O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+
-
 Burkholderia pseudomallei 1106a O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 ? x * 25000, recombinant His-tagged enzyme, SDS-PAGE Burkholderia pseudomallei

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Fe-SOD
-
 Burkholderia pseudomallei
1.15.1.1 iron-containing superoxide dismutase
-
 Burkholderia pseudomallei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 37
-
-
 Burkholderia pseudomallei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 37
-
 purified recombinant His-tagged enzyme, 48 h, over 80% activity remaining Burkholderia pseudomallei
1.15.1.1 50
-
 purified recombinant His-tagged enzyme, completely stable for 6 h, after 48 h 30% activity remaining Burkholderia pseudomallei
1.15.1.1 70
-
 purified recombinant His-tagged enzyme, 20% activity remaining after 1 h, inactivation after 3 h Burkholderia pseudomallei
1.15.1.1 100
-
 purified recombinant His-tagged enzyme, 5% activity remaining after 1 h, inactivation after 2 h Burkholderia pseudomallei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 8
-
-
 Burkholderia pseudomallei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 7 12 over 70% of maximal activity within this range Burkholderia pseudomallei