EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.7.4 | expressed in Escherichia coli as a His-tagged fusion protein | Burkholderia glumae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.7.4 | 0.006 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae | |
1.17.7.4 | 0.0212 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.7.4 | Fe2+ | iron content of BgHDR1 is determined as 1.6 Fe atoms per protein | Burkholderia glumae | |
1.17.7.4 | Fe2+ | iron content of BgHDR1 is determined as 1.7 Fe atoms per protein | Burkholderia glumae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 49900 | - |
calculated from cDNA | Burkholderia glumae |
1.17.7.4 | 51330 | - |
calculated from cDNA | Burkholderia glumae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.7.4 | Burkholderia glumae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.7.4 | using Ni-NTA chromatography | Burkholderia glumae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NADPH | - |
Burkholderia glumae | dimethylallyl diphosphate + isopentenyl diphosphate + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.7.4 | (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase | - |
Burkholderia glumae |
1.17.7.4 | BgHDR1 | - |
Burkholderia glumae |
1.17.7.4 | BgHDR2 | - |
Burkholderia glumae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.7.4 | 1.11 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae | |
1.17.7.4 | 3.11 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.7.4 | 8 | - |
assay at | Burkholderia glumae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.7.4 | NADPH | - |
Burkholderia glumae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.17.7.4 | malfunction | HDR1 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) | Burkholderia glumae |
1.17.7.4 | malfunction | HDR2 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) | Burkholderia glumae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.7.4 | 52.35 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae | |
1.17.7.4 | 518.3 | - |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate | pH 8, temperature not specified in the publication | Burkholderia glumae |