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Literature summary extracted from

  • Kwon, M.; Shin, B.; Lee, J.; Han, J.; Kim, S.
    Characterization of Burkholderia glumae BGR1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), the terminal enzyme in 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway (2013), J. Korean Soc. Appl. Biol. Chem., 56, 35-40.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.4 expressed in Escherichia coli as a His-tagged fusion protein Burkholderia glumae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.7.4 0.006
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae
1.17.7.4 0.0212
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.4 Fe2+ iron content of BgHDR1 is determined as 1.6 Fe atoms per protein Burkholderia glumae
1.17.7.4 Fe2+ iron content of BgHDR1 is determined as 1.7 Fe atoms per protein Burkholderia glumae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.7.4 49900
-
calculated from cDNA Burkholderia glumae
1.17.7.4 51330
-
calculated from cDNA Burkholderia glumae

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.4 Burkholderia glumae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.7.4 using Ni-NTA chromatography Burkholderia glumae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NADPH
-
Burkholderia glumae dimethylallyl diphosphate + isopentenyl diphosphate + NADP+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
-
Burkholderia glumae
1.17.7.4 BgHDR1
-
Burkholderia glumae
1.17.7.4 BgHDR2
-
Burkholderia glumae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.7.4 1.11
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae
1.17.7.4 3.11
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.7.4 8
-
assay at Burkholderia glumae

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.7.4 NADPH
-
Burkholderia glumae

General Information

EC Number General Information Comment Organism
1.17.7.4 malfunction HDR1 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) Burkholderia glumae
1.17.7.4 malfunction HDR2 is able to complement an Escherichia coli hdr deletion mutant (DYTL1) Burkholderia glumae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.7.4 52.35
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae
1.17.7.4 518.3
-
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8, temperature not specified in the publication Burkholderia glumae