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Literature summary extracted from

  • Li, X.; Yu, H.Y.
    Halostable cellulase with organic solvent tolerance from Haloarcula sp. LLSG7 and its application in bioethanol fermentation using agricultural wastes (2013), J. Ind. Microbiol. Biotechnol., 40, 1357-1365.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 biofuel production bioethanol fermentation using agricultural wastes Haloarcula sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Haloarcula sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 NaCl no activity in absence of NaCl, optimal activity of crude enzyme at 20% NaCl Haloarcula sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 1-butanol 25% (v/v), 30°C, 10 min, 56.1% residual activity Haloarcula sp.
3.2.1.4 1-decanol 25% (v/v), 30°C, 10 min, 81.8% residual activity Haloarcula sp.
3.2.1.4 Acetone 25% (v/v), 30°C, 10 min, 47.3% residual activity Haloarcula sp.
3.2.1.4 acetonitrile 25% (v/v), 30°C, 10 min, 51.8% residual activity Haloarcula sp.
3.2.1.4 benzene 25% (v/v), 30°C, 10 min, 127.9% residual activity Haloarcula sp.
3.2.1.4 chloroform 25% (v/v), 30°C, 10 min, 72.5% residual activity Haloarcula sp.
3.2.1.4 cyclohexane 25% (v/v), 30°C, 10 min, 75.0% residual activity Haloarcula sp.
3.2.1.4 dimethyl formamide 25% (v/v), 30°C, 10 min, 58.1% residual activity Haloarcula sp.
3.2.1.4 DMSO 25% (v/v), 30°C, 10 min, 41.2% residual activity Haloarcula sp.
3.2.1.4 Ethanol 25% (v/v), 30°C, 10 min, 35.2% residual activity Haloarcula sp.
3.2.1.4 isooctane 25% (v/v), 30°C, 10 min, 79.9% residual activity Haloarcula sp.
3.2.1.4 Methanol 25% (v/v), 30°C, 10 min, 38.1% residual activity Haloarcula sp.
3.2.1.4 n-hexane 25% (v/v), 30°C, 10 min, 126.1% residual activity Haloarcula sp.
3.2.1.4 toluene 25% (v/v), 30°C, 10 min, 84.1% residual activity Haloarcula sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Haloarcula sp.
-
-
-
3.2.1.4 Haloarcula sp. LLSG7
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellulose + H2O maximal activity soluble cellulosic substrate (CMC-Na salt), indicating that the crude enzyme has endoglucanase activity Haloarcula sp. ?
-
 ?
3.2.1.4 cellulose + H2O maximal activity soluble cellulosic substrate (CMC-Na salt), indicating that the crude enzyme has endoglucanase activity Haloarcula sp. LLSG7 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
 crude enzyme Haloarcula sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 20 80 20°C: about 40% of maximal activity, 80°C: about 70% of maximal activity, crude enzyme Haloarcula sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 8
-
 crude enzyme Haloarcula sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 7 11 pH 7.0: about 85% of maximal activity, pH 11.0: about 70% of maximal activity Haloarcula sp.