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Literature summary extracted from

  • Yu, Z.; Liu, Y.; Li, Z.
    Structure-function relationship of the Polo-like kinase in Trypanosoma brucei (2012), J. Cell Sci., 125, 1519-1530.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.21 the Trypanosoma brucei EYFP-tagged enzyme is ectopically expressed in HeLa cells. It is predominantly nuclear in interphase cells, whereas in mitotic cells it is localized throughout the cytoplasm. It is unable to be targeted to spindle poles, the central spindle or the midbody in HeLa cells Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.21 F561A mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei
2.7.11.21 H710A mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei
2.7.11.21 K70R no kinase activity Trypanosoma brucei
2.7.11.21 K712A mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei
2.7.11.21 additional information deletion of the C-terminal polo-box domain abolishes localization of polo-like kinase Trypanosoma brucei
2.7.11.21 T198A mutation disrupts the ability to phosphorylate the in vitro substrate TbCentrin2 Trypanosoma brucei
2.7.11.21 T198D kinase activity of the mutant enzyme is several-fold higher than that of wild-type enzyme Trypanosoma brucei
2.7.11.21 T201A mutation disrupts the ability to phosphorylate the in vitro substrate TbCentrin2 Trypanosoma brucei
2.7.11.21 T201D kinase activity of the mutant enzyme is several-fold higher than that of wild-type enzyme Trypanosoma brucei
2.7.11.21 W557F mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.21 flagellum the enzyme is enriched at the anterior tip of the flagellum attachment zone Trypanosoma brucei
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.21 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.21 ATP + protein p110
-
 Trypanosoma brucei ADP + ?
-
 ?
2.7.11.21 ATP + TbCentrin2
-
 Trypanosoma brucei ADP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.21 TbPLK
-
 Trypanosoma brucei