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Literature summary extracted from

  • Sugii, T.; Akanuma, S.; Yagi S.; Yagyu, K.; Shimoda, Y.; Yamagishi, A.
    Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants (2014), J. Biosci. Bioeng., 118, 367-371.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.119 expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.119 V254I mutant has improved kcat and kcat/Km values at both 25°C and 80°C. The thermal stability of the mutant enzyme was comparable to that of the wild-type enzyme. Calculation of the energetic contribution of the V254I mutation for the dehydrogenase reaction reveals that the mutation destabilizes the enzyme-NADPD-glucose ternary complex and reduces the transition-state energy, thus enhancing catalysis Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.119 0.00588
-
 NADP+ pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.00876
-
 NADP+ pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.00927
-
 NADP+ pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.0286
-
 D-glucose pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.0293
-
 D-glucose pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.0391
-
 D-glucose pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.109
-
 NADP+ pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.117
-
 NADP+ pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.132
-
 NADP+ pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.287
-
 D-glucose pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.314
-
 D-glucose pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.457
-
 D-glucose pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.119 D-glucose + NADP+ Sulfurisphaera tokodaii
-
 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.119 D-glucose + NADP+ Sulfurisphaera tokodaii DSM 16993
-
 D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.119 Sulfurisphaera tokodaii Q96ZY7
-
-
1.1.1.119 Sulfurisphaera tokodaii DSM 16993 Q96ZY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.119
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.119 D-glucose + NADP+
-
 Sulfurisphaera tokodaii D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.119 D-glucose + NADP+ the enzyme prefers NADP+ over NAD+ as the cofactor. It shows a larger Km value for NAD+ (4.5 mM) than for NADP+ (0.12 mM) at 80°C. At 25°C the Km values are 0.069 mM for NAD+ and 0.0059 mM for NADP+ Sulfurisphaera tokodaii D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.119 D-glucose + NADP+
-
 Sulfurisphaera tokodaii DSM 16993 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.119 D-glucose + NADP+ the enzyme prefers NADP+ over NAD+ as the cofactor. It shows a larger Km value for NAD+ (4.5 mM) than for NADP+ (0.12 mM) at 80°C. At 25°C the Km values are 0.069 mM for NAD+ and 0.0059 mM for NADP+ Sulfurisphaera tokodaii DSM 16993 D-glucono-1,5-lactone + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.119 GDH
-
 Sulfurisphaera tokodaii
1.1.1.119 STK_17040 locus name Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.119 0.085
-
 D-glucose pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.085
-
 NADP+ pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.11
-
 D-glucose pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.11
-
 NADP+ pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.205
-
 D-glucose pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.205
-
 NADP+ pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 9.7
-
 D-glucose pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 9.7
-
 NADP+ pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 10.1
-
 D-glucose pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 10.1
-
 NADP+ pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 16.7
-
 D-glucose pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 16.7
-
 NADP+ pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.119 9
-
 assay at Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.119 NADP+ the enzyme prefers NADP+ over NAD+ as the cofactor. It shows a larger Km value for NAD+ (4.5 mM) than for NADP+ (0.12 mM) at 80°C. At 25°C the Km values are 0.069 mM for NAD+ and 0.0059 mM for NADP+ Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.119 0.092
-
 NADP+ pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.126
-
 NADP+ pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.186
-
 NADP+ pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.234
-
 NADP+ pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.31
-
 D-glucose pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 0.351
-
 D-glucose pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.365
-
 D-glucose pH 9.0, 80°C, mutant enzyme V254I Sulfurisphaera tokodaii
1.1.1.119 0.863
-
 NADP+ pH 9.0, 80°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 0.893
-
 NADP+ pH 9.0, 80°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 2.91
-
 D-glucose pH 9.0, 25°C, mutant enzyme I189V Sulfurisphaera tokodaii
1.1.1.119 3.82
-
 D-glucose pH 9.0, 25°C, wild-type enzyme Sulfurisphaera tokodaii
1.1.1.119 5.25
-
 D-glucose pH 9.0, 25°C, mutant enzyme V254I Sulfurisphaera tokodaii