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Literature summary extracted from

  • Miura, H.; Hori, K.; Sasaki, Y.; Inahashi, Y.; Yagisawa, Y.; Fujita, N.; Omura, S.; Takahashi, Y.
    Simple analytic method of diaminopimelate epimerase activity (2013), J. Biosci. Bioeng., 116, 253-255.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.1.7 drug development the enzme is an attractive target to facilitate development of antibacterial agents Kitasatospora setae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.7 gene dapF1, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS/pET15b Kitasatospora setae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.7 acetone inactivation by 50% acetone Kitasatospora setae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.7 LL-2,6-Diaminoheptanedioate Kitasatospora setae
-
 meso-Diaminoheptanedioate
-
 r
5.1.1.7 LL-2,6-Diaminoheptanedioate Kitasatospora setae KM-6054
-
 meso-Diaminoheptanedioate
-
 r

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
5.1.1.7 Acetone inactivation by 50% acetone Kitasatospora setae

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.7 Kitasatospora setae E4NI20 gene dapF1; gene dapF1
-
5.1.1.7 Kitasatospora setae KM-6054 E4NI20 gene dapF1; gene dapF1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.7 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS/pET15b by nickel affinity chromatography Kitasatospora setae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.7 1.61
-
 purified recombinant His-tagged enzyme, substrate meso-diaminoheptanedioate, pH 6.8, 30°C Kitasatospora setae
5.1.1.7 4.93
-
 purified recombinant His-tagged enzyme, substrate LL-2,6-diaminoheptanedioate, pH 6.8, 30°C Kitasatospora setae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.7 LL-2,6-Diaminoheptanedioate
-
 Kitasatospora setae meso-Diaminoheptanedioate
-
 r
5.1.1.7 LL-2,6-Diaminoheptanedioate
-
 Kitasatospora setae KM-6054 meso-Diaminoheptanedioate
-
 r
5.1.1.7 additional information no activity with DD-2,6-diaminoheptanedioate. Development of a simple method using thin-layer chromatography, in methanol/water (64:36) and with ninhydrin detection, and chiral column chromatography to allow preparation of pure diaminopimelate isomers and detect products, respectively, overview Kitasatospora setae ?
-
 ?
5.1.1.7 additional information no activity with DD-2,6-diaminoheptanedioate. Development of a simple method using thin-layer chromatography, in methanol/water (64:36) and with ninhydrin detection, and chiral column chromatography to allow preparation of pure diaminopimelate isomers and detect products, respectively, overview Kitasatospora setae KM-6054 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.7 DAP epimerase
-
 Kitasatospora setae
5.1.1.7 DapF
-
 Kitasatospora setae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.7 30
-
 assay at Kitasatospora setae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.7 6.8
-
 assay at Kitasatospora setae

General Information

EC Number General Information Comment Organism
5.1.1.7 physiological function diaminopimelate isomers are not only intermediates of the lysine biosynthesis diaminopimelate pathway but also essential components of bacterial peptidoglycan Kitasatospora setae