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Literature summary extracted from

  • Morimoto, K.; Terami, Y.; Maeda, Y.; Yoshihara, A.; Takata, G.; Izumori, K.
    Cloning and characterization of the L-ribose isomerase gene from Cellulomonas parahominis MB426 (2013), J. Biosci. Bioeng., 115, 377-381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.B3 expressed in Escherichia coli JM109 cells Cellulomonas parahominis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.B3 Ag+
-
 Cellulomonas parahominis
5.3.1.B3 Ba2+
-
 Cellulomonas parahominis
5.3.1.B3 Ca2+
-
 Cellulomonas parahominis
5.3.1.B3 Cu2+
-
 Cellulomonas parahominis
5.3.1.B3 Fe2+
-
 Cellulomonas parahominis
5.3.1.B3 Li+
-
 Cellulomonas parahominis
5.3.1.B3 Ni2+
-
 Cellulomonas parahominis
5.3.1.B3 Zn2+
-
 Cellulomonas parahominis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.B3 15.4
-
 L-ribulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 30.4
-
 L-ribose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 32.8
-
 D-xylulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 37.1
-
 D-Lyxose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 37.6
-
 D-talose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 39.2
-
 D-tagatose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 40
-
 L-sorbose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 40.1
-
 L-gulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 40.3
-
 L-allose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 51
-
 D-fructose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 51.1
-
 D-mannose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 54.8
-
 L-psicose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.B3 additional information the enzyme is not dependent for metallic ions for its activity Cellulomonas parahominis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.B3 27435
-
 x * 27435, calculated from amino acid sequence Cellulomonas parahominis
5.3.1.B3 32000
-
 x * 32000, SDS-PAGE Cellulomonas parahominis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.B3 L-ribose Cellulomonas parahominis highest activity with L-ribose L-ribulose
-
 r
5.3.1.B3 L-ribose Cellulomonas parahominis MB426 highest activity with L-ribose L-ribulose
-
 r
5.3.1.B3 L-ribulose Cellulomonas parahominis
-
 L-ribose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.B3 Cellulomonas parahominis L0N3Y0
-
-
5.3.1.B3 Cellulomonas parahominis MB426 L0N3Y0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.B3 ammonium sulfate precipitation, Q Sepharose column chromatography, Resource PHE column chromatographym and Resource Q column chromatography Cellulomonas parahominis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.B3 1.49
-
 crude native enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 4.9
-
 crude recombinant enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 39.5
-
 native enzyme after 27fold purification, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 214
-
 recombinant enzyme after 43.6fold purification, in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.B3 D-fructose
-
 Cellulomonas parahominis D-mannose
-
 r
5.3.1.B3 D-Lyxose 43.4% activity compared to L-ribose Cellulomonas parahominis D-Xylulose
-
 r
5.3.1.B3 D-Lyxose 43.4% activity compared to L-ribose Cellulomonas parahominis MB426 D-Xylulose
-
 r
5.3.1.B3 D-Mannose 2.07% activity compared to L-ribose Cellulomonas parahominis D-Fructose
-
 r
5.3.1.B3 D-Mannose 2.07% activity compared to L-ribose Cellulomonas parahominis MB426 D-Fructose
-
 r
5.3.1.B3 D-tagatose
-
 Cellulomonas parahominis D-talose
-
 r
5.3.1.B3 D-talose 36.8% activity compared to L-ribose Cellulomonas parahominis D-tagatose
-
 r
5.3.1.B3 D-talose 36.8% activity compared to L-ribose Cellulomonas parahominis MB426 D-tagatose
-
 r
5.3.1.B3 D-xylulose
-
 Cellulomonas parahominis D-lyxose
-
 r
5.3.1.B3 L-allose 8.34% activity compared to L-ribose Cellulomonas parahominis L-psicose
-
 r
5.3.1.B3 L-allose 8.34% activity compared to L-ribose Cellulomonas parahominis MB426 L-psicose
-
 r
5.3.1.B3 L-gulose 2.27% activity compared to L-ribose Cellulomonas parahominis L-sorbose
-
 r
5.3.1.B3 L-psicose
-
 Cellulomonas parahominis L-allose
-
 r
5.3.1.B3 L-ribose highest activity with L-ribose Cellulomonas parahominis L-ribulose
-
 r
5.3.1.B3 L-ribose highest activity with L-ribose Cellulomonas parahominis MB426 L-ribulose
-
 r
5.3.1.B3 L-ribulose
-
 Cellulomonas parahominis L-ribose
-
 r
5.3.1.B3 L-sorbose
-
 Cellulomonas parahominis L-gulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.B3 ? x * 32000, SDS-PAGE Cellulomonas parahominis
5.3.1.B3 ? x * 27435, calculated from amino acid sequence Cellulomonas parahominis

Synonyms

EC Number Synonyms Comment Organism
5.3.1.B3 LRI
-
 Cellulomonas parahominis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.B3 40
-
-
 Cellulomonas parahominis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.B3 40
-
 the enzyme is stable up to 40°C for 1 h Cellulomonas parahominis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.B3 0.38
-
 L-sorbose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 2.1
-
 L-gulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 2.2
-
 L-psicose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 2.5
-
 D-mannose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 2.8
-
 L-allose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 15.8
-
 D-talose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 17.3
-
 D-Lyxose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 17.5
-
 D-fructose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 18.2
-
 D-xylulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 40.8
-
 D-tagatose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 95.2
-
 L-ribose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 108.8
-
 L-ribulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.B3 9
-
-
 Cellulomonas parahominis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.B3 6 11 more than 60% activity between pH 6.0 and 11.0 Cellulomonas parahominis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.B3 7 10 the enzyme is stable in a pH range of pH 7.0-10.0 at 4°C for 24 h in any buffer Cellulomonas parahominis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.B3 0.009
-
 L-sorbose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.034
-
 D-fructose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.04
-
 L-psicose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.048
-
 D-mannose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.054
-
 L-gulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.07
-
 L-allose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.104
-
 D-tagatose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.42
-
 D-talose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.47
-
 D-Lyxose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 0.56
-
 D-xylulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 3.13
-
 L-ribose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis
5.3.1.B3 7.05
-
 L-ribulose in 50 mM glycine-NaOH buffer (pH 9.0), at 40°C Cellulomonas parahominis