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Literature summary extracted from

  • Hirota, R.; Yamane, S.T.; Fujibuchi, T.; Motomura, K.; Ishida, T.; Ikeda, T.; Kuroda, A.
    Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506 (2012), J. Biosci. Bioeng., 113, 445-450.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.1.1 expression in Escherichia coli Pseudomonas stutzeri
1.20.1.1 expression in Escherichia coli Ralstonia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.20.1.1 arsenite 4 mM, 90% residual activity Pseudomonas stutzeri
1.20.1.1 additional information not inhibitory: arsenite, NaCl Ralstonia sp.
1.20.1.1 NaCl 4 mM, 87% residual activity Pseudomonas stutzeri
1.20.1.1 nitrate 4 mM, 21% residual activity Pseudomonas stutzeri
1.20.1.1 nitrate 4 mM, 40% residual activity Ralstonia sp.
1.20.1.1 sulfite 4 mM, 16% residual activity Pseudomonas stutzeri
1.20.1.1 sulfite 4 mM, 56% residual activity Ralstonia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.1.1 0.0026
-
 NAD+ pH 7.2, 30°C Ralstonia sp.
1.20.1.1 0.0073
-
 phosphonate pH 7.2, 30°C Pseudomonas stutzeri
1.20.1.1 0.0087
-
 phosphonate pH 7.2, 30°C Ralstonia sp.
1.20.1.1 0.016
-
 NAD+ pH 7.2, 40°C Ralstonia sp.
1.20.1.1 0.024
-
 phosphonate pH 7.2, 40°C Ralstonia sp.
1.20.1.1 0.056
-
 NAD+ pH 7.2, 30°C Pseudomonas stutzeri
1.20.1.1 0.128
-
 NAD+ pH 7.2, 40°C Pseudomonas stutzeri
1.20.1.1 0.287
-
 phosphonate pH 7.2, 40°C Pseudomonas stutzeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.20.1.1 soluble recombinant protein Ralstonia sp.
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.20.1.1 Pseudomonas stutzeri
-
-
-
1.20.1.1 Pseudomonas stutzeri WM88
-
-
-
1.20.1.1 Ralstonia sp. G4XDR8
-
-
1.20.1.1 Ralstonia sp. 4506 G4XDR8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.1.1 phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri phosphate + NADH + H+
-
 ?
1.20.1.1 phosphonate + NAD+ + H2O
-
 Ralstonia sp. phosphate + NADH + H+
-
 ?
1.20.1.1 phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri WM88 phosphate + NADH + H+
-
 ?
1.20.1.1 phosphonate + NAD+ + H2O
-
 Ralstonia sp. 4506 phosphate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.20.1.1 PtxD
-
 Pseudomonas stutzeri
1.20.1.1 PtxD
-
 Ralstonia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.1.1 50
-
-
 Pseudomonas stutzeri
1.20.1.1 50
-
-
 Ralstonia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.20.1.1 45
-
 half-life 80.5 h Pseudomonas stutzeri
1.20.1.1 45
-
 half-life 80.5 h Ralstonia sp.