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Literature summary extracted from

  • Touhara, K.K.; Nihira, T.; Kitaoka, M.; Nakai1, H.; Fushinobu, S.
    Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-glucosylglycerol phosphorylase (2014), J. Biol. Chem., 289, 18067-18075.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.332 expressed in Escherichia coli Salisediminibacterium selenitireducens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.332 obtained at 20ΒΊC using the hanging drop vapor diffusion method by mixing 1 microL of protein solution containing 4.3Β–5.7 mg/mL protein with an equal volume of reservoir solution containing 28% polyethylene glycol 400, 0.1 M HEPES-NaOH (pH 7.5), and 0.2 M CaCl2 Salisediminibacterium selenitireducens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.332 E475Q inactive mutant Salisediminibacterium selenitireducens
2.4.1.332 K587A inactive mutant Salisediminibacterium selenitireducens
2.4.1.332 W381F inactive mutant Salisediminibacterium selenitireducens
2.4.1.332 Y327F inactive mutant Salisediminibacterium selenitireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens
-
 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens DSM 15326
-
 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens DSM 15326
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.332 Salisediminibacterium selenitireducens D6XZ22
-
-
2.4.1.332 Salisediminibacterium selenitireducens DSM 15326 D6XZ22
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 Salisediminibacterium selenitireducens beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid Salisediminibacterium selenitireducens beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 Salisediminibacterium selenitireducens DSM 15326 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid Salisediminibacterium selenitireducens DSM 15326 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 ?
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 ?
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
2.4.1.332 additional information in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule Salisediminibacterium selenitireducens ?
-
 ?
2.4.1.332 additional information in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule Salisediminibacterium selenitireducens DSM 15326 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.332 2-O-alpha-D-glucopyranosylglycerol phosphorylase
-
 Salisediminibacterium selenitireducens
2.4.1.332 Bsel_2816 locus name Salisediminibacterium selenitireducens