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Literature summary extracted from

  • Lee, M.J.; Gravelat, F.N.; Cerone, R.P.; Baptista, S.D.; Campoli, P.V.; Choe, S.I.; Kravtsov, I.; Vinogradov, E.; Creuzenet, C.; Liu, H.; Berghuis, A.M.; Latge, J.P.; Filler, S.G.; Fontaine, T.; Sheppard, D.C.
    Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides (2014), J. Biol. Chem., 289, 1243-1256.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 genes uge3 and uge5, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), expression as fluorescence labeled proteins uge3-gfp and uge5-rfp in Aspergillus. fumigatus strain Af293 Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.2 additional information generation of single disruption mutants and DELTAuge3 DELTAuge5 double mutant, phenotypes, overview Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.3.2 cell wall
-
 Aspergillus fumigatus 5618
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.2 UDP-alpha-D-glucose Aspergillus fumigatus
-
 UDP-alpha-D-galactose
-
 ?
5.1.3.2 UDP-alpha-D-glucose Aspergillus fumigatus Af293
-
 UDP-alpha-D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Aspergillus fumigatus
-
 genes uge3 and uge5 encoding isozymes Uge3 and Uge5
-
5.1.3.2 Aspergillus fumigatus Af293
-
 genes uge3 and uge5 encoding isozymes Uge3 and Uge5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2 recombinant His6-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.2 conidium
-
 Aspergillus fumigatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-alpha-D-glucose
-
 Aspergillus fumigatus UDP-alpha-D-galactose
-
 ?
5.1.3.2 UDP-alpha-D-glucose
-
 Aspergillus fumigatus Af293 UDP-alpha-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.2 More homology structural modeling, overview Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 UGE3
-
 Aspergillus fumigatus
5.1.3.2 UGE5
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.2 37
-
 assay at Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.2 8
-
 assay at Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
5.1.3.2 malfunction In the absence of Uge5, Uge3 activity is sufficient for growth on galactose and the synthesis of galactosaminogalactan containing lower levels of galactose but not the synthesis of galactofuranose. A double deletion of uge5 and uge3 blocked growth on galactose and synthesis of both galactofuranose and galactosaminogalactan, phenotypes, overview Aspergillus fumigatus
5.1.3.2 metabolism overlapping and distinct roles of UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides, galactosaminogalactan synthesis requires the UDP-glucose 4-epimerases, Uge5 and Uge3, whereas galactomannan synthesis requires Uge5 alone, overview Aspergillus fumigatus
5.1.3.2 additional information homology structural modeling, overview Aspergillus fumigatus
5.1.3.2 physiological function Uge5 is the dominant UDP-glucose 4-epimerase in Aspergillus fumigatus and is essential for normal growth in galactose-based medium. Uge5 is required for synthesis of the galactofuranose component of galactomannan and contributes galactose to the synthesis of galactosaminogalactan. Uge3 can mediate production of both UDP-galactose and UDP-N-acetylgalactosamine, cf. EC 5.1.3.7, and is required for the production of galactosaminogalactan but not galactomannan Aspergillus fumigatus