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Literature summary extracted from

  • Huo, L.; Davis, I.; Chen, L.; Liu, A.
    The power of two: arginine 51 and arginine 239* from a neighboring subunit are essential for catalysis in alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (2013), J. Biol. Chem., 288, 30862-30871.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.45 expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.45 R239A catalytically inactive Pseudomonas fluorescens
4.1.1.45 R239K catalytically inactive Pseudomonas fluorescens
4.1.1.45 R51A catalytically inactive Pseudomonas fluorescens
4.1.1.45 R51K catalytically inactive Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.45 pyridine-2,6-dicarboxylic acid competitive Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.45 0.0096
-
 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate wild type enzyme, at pH 7.0 and 37°C Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate Pseudomonas fluorescens
-
 2-aminomuconate semialdehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.45 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.45 Superdex 75 gel filtration Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.45 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate
-
 Pseudomonas fluorescens 2-aminomuconate semialdehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.45 homodimer
-
 Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.45 ACMSD
-
 Pseudomonas fluorescens
4.1.1.45 alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase
-
 Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.45 6.5
-
 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate wild type enzyme, at pH 7.0 and 22°C Pseudomonas fluorescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.45 0.0227
-
 pyridine-2,6-dicarboxylic acid wild type enzyme, at pH 7.0 and 22°C Pseudomonas fluorescens