EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.24 | expressed in Escherichia coli Top10 cells | Rhodococcus jostii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.13.24 | sitting drop vapor diffusion method, using 28% (w/v) PEG 4000, 0.2 M sodium acetate, and 0.1 M Tris-HCl (pH 8.5) | Rhodococcus jostii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.24 | H213A | inactive, but the strength of substrate binding is not affected by the mutation | Rhodococcus jostii |
1.14.13.24 | H213S | inactive | Rhodococcus jostii |
1.14.13.24 | Q301E | inactive | Rhodococcus jostii |
1.14.13.24 | Y105F | the mutant shows less affinity for the aromatic substrate and lower catalytic efficiency compared to the wild type enzyme | Rhodococcus jostii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.24 | 0.027 | - |
3-hydroxybenzoate | wild type enzyme, at pH 8.0 and 25°C | Rhodococcus jostii | |
1.14.13.24 | 0.186 | - |
3-hydroxybenzoate | mutant enzyme Y105F, at pH 8.0 and 25°C | Rhodococcus jostii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.24 | 3-hydroxybenzoate + NADH + H+ + O2 | Rhodococcus jostii | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.24 | Rhodococcus jostii | Q0SFK6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.24 | 3-hydroxybenzoate + NADH + H+ + O2 | - |
Rhodococcus jostii | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.24 | homodimer | x-ray crystallography | Rhodococcus jostii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.24 | 3-hydroxybenzoate 6-hydroxylase | - |
Rhodococcus jostii |
1.14.13.24 | 3HB6H | - |
Rhodococcus jostii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.24 | 3 | 6 | 3-hydroxybenzoate | wild type enzyme, at pH 8.0 and 25°C | Rhodococcus jostii | |
1.14.13.24 | 41 | - |
3-hydroxybenzoate | mutant enzyme Y105F, at pH 8.0 and 25°C | Rhodococcus jostii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.24 | FAD | each subunit contains a tightly but non-covalently bound FAD | Rhodococcus jostii | |
1.14.13.24 | NADH | - |
Rhodococcus jostii |