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Literature summary extracted from

  • Raedts, J.; Lundgren, M.; Kengen, S.W.; Li, J.P.; van der Oost, J.
    A novel bacterial enzyme with D-glucuronyl C5-epimerase activity (2013), J. Biol. Chem., 288, 24332-24339.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.17 gene RED65_08024, design and synthesis of a synthetic based on hypothetical protein RED65_08024, codon optimized for the codon usage bias of Escherichia coli, expression of the recombinant His-tagged enzyme in Escherichia coli strain Rossetta(DE3)/pWUR537 Bermanella marisrubri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.17 51914
-
 x * 51914, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE Bermanella marisrubri
5.1.3.17 52000
-
 x * 51914, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE Bermanella marisrubri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.17 Heparosan-N-sulfate D-glucuronate Bermanella marisrubri
-
 Heparosan-N-sulfate L-iduronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.17 Bermanella marisrubri Q1N097
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.17 recombinant His-tagged enzyme from Escherichia coli strain Rossetta(DE3)/pWUR537 by cobalt affinity chromatography Bermanella marisrubri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.17 Heparosan-N-sulfate D-glucuronate
-
 Bermanella marisrubri Heparosan-N-sulfate L-iduronate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.17 dimer or tetramer x * 51914, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE Bermanella marisrubri
5.1.3.17 More RED-C5-epimerase is present in a dimeric/tetrameric form, in approximately a 2:1 ratio, and appears to be in a dynamic equilibrium Bermanella marisrubri

Synonyms

EC Number Synonyms Comment Organism
5.1.3.17 D-glucuronyl C5-epimerase
-
 Bermanella marisrubri
5.1.3.17 RED-C5-epimerase
-
 Bermanella marisrubri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.17 30
-
 assay at Bermanella marisrubri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.17 37
-
 purified recombinant His-tagged enzyme, inactivation Bermanella marisrubri

General Information

EC Number General Information Comment Organism
5.1.3.17 metabolism the enzyme is probably involved in bacterial capsular polysaccharide biosynthesis, it shows catalytic properties similar to murine heparan sulfate D-glucuronyl C5-epimerase. The biosynthesis of these complex polysaccharides involves complicated reactions that turn the simple glycosaminoglycan backbone into highly heterogeneous structures. One of the modification reactions is the epimerization of D-glucuronic acid to its C5-epimer L-iduronic acid, which is essential for the function of heparan sulfate Bermanella marisrubri