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Literature summary extracted from

  • Lundqvist, J.; Braumann, I.; Kurowska, M.; Mueller, A.H.; Hansson, M.
    Catalytic turnover triggers exchange of subunits of the magnesium chelatase AAA+ motor unit (2013), J. Biol. Chem., 288, 24012-24019.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.6.1.1 expressed in Escherichia coli as a His-tagged fusion protein Rhodobacter capsulatus

Protein Variants

EC Number Protein Variants Comment Organism
6.6.1.1 D207N BchI mutant forms a complex with wild-type BchD. Mutant shows 7.2% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity Rhodobacter capsulatus
6.6.1.1 L111F BchI mutant forms a complex with wild-type BchD. Mutant shows 5.5% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity Rhodobacter capsulatus
6.6.1.1 additional information mixing experiments with modified and wild-type BchI in various combinations show that an exchange of BchI subunits in magnesium chelatase occurs during the catalytic cycle, indicating that a dissociation of the complex may be part of the reaction mechanism Rhodobacter capsulatus
6.6.1.1 R289K BchI mutant forms a complex with wild-type BchD. Mutant shows 10.9% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity Rhodobacter capsulatus
6.6.1.1 V113G BchI mutant forms a complex with wild-type BchD. Mutant shows 25% of wild-type ATPase activity. Mutant shows magnesium chelatase activity. Magnesium chelatase assays containing a mixture of mutant BchI V113G and wild-type BchI have the effect of stimulating the activity Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Rhodobacter capsulatus
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.6.1.1 using Ni-NTA chromatography Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + deuteroporphyrin IX + Mg2+ + H2O
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 Rhodobacter capsulatus ADP + phosphate + Mg-deuteroporphyrin IX + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.6.1.1 BchID
-
 Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.6.1.1 22
-
 assay at Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6.1.1 8.5
-
 assay at Rhodobacter capsulatus