EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.3.99.3 | dithiothreitol | leads to dissociation of the bound heme which is hindered by imidazole | Macaca fascicularis | |
5.3.99.3 | GSH | mPGES2 has a GSH-binding motif, the GSH molecule binds at the predicted amino acid residues 148VPxL to 164DSxxI. GSH is essential for heme binding to isozyme mPGES2, structures of GSH-heme complex-binding cavity in isozyme mPGES2, overview | Macaca fascicularis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.99.3 | heme | heme is bound to the enzyme subunits and alters the enzyme catalytic specificity, overview. Heme is attached to the bound GSH by an iron-sulfur bond, but heme cannot bind to mPGES2 in the absence of GSH, other sulfhydryl compounds, such as DTT and 2-mercaptoethanol, are unable to promote heme binding to isozyme mPGES2. Bound heme is oxidized by H2O2, but it does not dissociate from mPGES2 | Macaca fascicularis | |
5.3.99.3 | imidazole | imidazole stacks on the bound heme and inhibits heme oxidation by H2O2, heme dissociation by DTT, and catalytic activity | Macaca fascicularis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.3.99.3 | membrane | membrane-associated prostaglandin E synthase-2 | Macaca fascicularis | 16020 | - |
5.3.99.3 | microsome | - |
Macaca fascicularis | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.99.3 | (5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | Macaca fascicularis | reaction of heme-free isozyme mPGES2 | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.99.3 | Macaca fascicularis | Q9N0A4 | isozyme mPGES2 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.3.99.3 | liver | - |
Macaca fascicularis | - |
5.3.99.3 | additional information | abundant tissue distribution of isozyme mPGES2 | Macaca fascicularis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.99.3 | (5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | reaction of heme-free isozyme mPGES2 | Macaca fascicularis | (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | - |
? | |
5.3.99.3 | additional information | heme-free isozyme mPGES2 and heme-bound isozyme mPGES2 catalyze isomerization of prostaglandin H2 to prostaglandin E2 and conversion of prostaglandin H2 to to (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoic acid and malondialdehyde, respectively | Macaca fascicularis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.99.3 | membrane-associated prostaglandin E synthase-2 | - |
Macaca fascicularis |
5.3.99.3 | MPGES2 | - |
Macaca fascicularis |
5.3.99.3 | prostaglandin E2 synthase | - |
Macaca fascicularis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.99.3 | 4 | - |
assay at | Macaca fascicularis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.99.3 | 7.2 | - |
assay at | Macaca fascicularis |
EC Number | Organism | Comment | Expression |
---|---|---|---|
5.3.99.3 | Macaca fascicularis | iron does not induce the enzyme expression | additional information |