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Literature summary extracted from

  • Tokgoez, Z.; Siepmann, T.J.; Streich, F.; Kumar, B.; Klein, J.M.; Haas, A.L.
    E1-E2 interactions in ubiquitin and Nedd8 ligation pathways (2012), J. Biol. Chem., 287, 311-321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.23
-
 Homo sapiens
6.2.1.64
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.23 DELTA946 truncation of the Uba3 carboxyl-terminal beta-grasp domain, no effect on cognate Ubc12 thiolester formation Homo sapiens
6.2.1.64 DELTA946 truncation of the Uba1 carboxyl-terminal beta-grasp domain reduces cognate Ubc2b binding by 31-fold and kcat by 33500-fold Homo sapiens
6.2.1.64 DELTA946 truncation of the Uba3 carboxyl-terminal beta-grasp domain, no effect on cognate Ubc12 thiolester formation Homo sapiens
6.2.1.64 additional information generation of a chimeric Ub1 isoform carrying the beta-grasp domain of Uba3. Ternary complex stoichiometries for wild type and chimeric Uba1 are identical showing that the beta-grasp domain is not required in the Uba1 catalytic cycle Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.64 0.0006
-
 ubiquitin mutant DELTA946, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.0008
-
 ubiquitin wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.0012
-
 ubiquitin chimeric Ub1, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.0045
-
 ATP chimeric Ub1, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.0054
-
 ATP mutant DELTA946, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.0055
-
 ATP wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.02
-
 ATP mutant DELTA348, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.103
-
 ATP wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.23 Homo sapiens P22314
-
-
2.3.2.23 Homo sapiens Q8TBC4 isoform Uba3
-
6.2.1.64 Homo sapiens P22314
-
-
6.2.1.64 Homo sapiens Q8TBC4 isoform Uba3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.23 additional information functional survey of 11 representative human E2 paralogs reveals similar Km values for binding to human Uba1 ternary complex with an average Km of 121 nM and kcat for ubiquitin transfer of 4.0 per s, suggesting that they possess a conserved binding site and transition state geometry and that they compete for charging through differences in intracellular concentration. This binding motif is localized to three basic residues within Helix 1 of the E2 core domain Homo sapiens ?
-
 ?
2.3.2.23 [ubiquitin-activating protein Uba1]-S-ubiquitinyl-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-L-cysteine
-
 Homo sapiens [ubiquitin-activating protein Uba1]-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-S-ubiquitinyl-L-cysteine
-
 ?
2.3.2.23 [ubiquitin-activating protein Uba3]-S-ubiquitinyl-L-cysteine + [ubiquitin-carrier-protein Ubc12]-L-cysteine
-
 Homo sapiens [ubiquitin-activating protein Uba3]-L-cysteine + [ubiquitin-carrier-protein Ubc12]-S-ubiquitinyl-L-cysteine
-
 ?
6.2.1.64 ATP + ubiquitin + [ubiquitin-activating protein UBA1]-L-cysteine
-
 Homo sapiens AMP + diphosphate + [ubiquitin-activating protein UBA1]-S-ubiquitinyl-L-cysteine
-
 ?
6.2.1.64 ATP + ubiquitin + [ubiquitin-activating protein Uba3]-L-cysteine
-
 Homo sapiens AMP + diphosphate + [ubiquitin-activating protein Uba3]-S-ubiquitinyl-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.23 NEDD8-activating enzyme E1 catalytic subunit
-
 Homo sapiens
2.3.2.23 Uba3
-
 Homo sapiens
6.2.1.64 NEDD8-activating enzyme E1 catalytic subunit
-
 Homo sapiens
6.2.1.64 Uba1
-
 Homo sapiens
6.2.1.64 Uba3
-
 Homo sapiens
6.2.1.64 ubiquitin-like modifier-activating enzyme 1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.64 0.000075
-
 ATP chimeric Ub1, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 0.00013
-
 ATP mutant DELTA946, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 3.1
-
 ATP mutant DELTA348, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 3.4
-
 ATP wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens
6.2.1.64 3.5
-
 ATP wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens