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Literature summary extracted from

  • Andres, E.; Martinez, N.; Planas, A.
    Expression and characterization of a Mycoplasma genitalium glycosyltransferase in membrane glycolipid biosynthesis: Potential target against Mycoplasma infections (2011), J. Biol. Chem., 286, 35367-35379.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.315 dioleoylphosphatidylglycerol kcat linearly increases with dioleoylphosphatidylglycerol concentration Mycoplasma genitalium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.315 expression in Escherichia coli Mycoplasma genitalium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.315 0.05
-
 UDP-alpha-D-glucose above 0.05, acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.087
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.09
-
 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.234
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.27
-
 1,2-diacyl-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.479
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.315 Mycoplasma genitalium Q9ZB73 ATCC 33530
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.315 additional information enzyme transfers glucosyl and galactosyl residues from UDP-Glc and UDP-Gal to dioleoylglycerol acceptor to form the corresponding beta-glycosyl-dioleoylglycerol, which then acts as acceptor to give beta-diglycosyl-dioleoylglycerol products. Enzyme sequentially adds glycosyl residues with beta-1,6 connectivity, the first glycosyltransferase activity to dioleoylglycerol being about 1 order of magnitude higher than the second to monoglucosyldioleoylglycerol Mycoplasma genitalium ?
-
 ?
2.4.1.315 UDP-alpha-D-galactose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 9% of the specific activity with 1,2-diacyl-sn-glycerol Mycoplasma genitalium 1,2-diacyl-3-O-[alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
2.4.1.315 UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 2.2% of the specific activity with 1,2-diacyl-sn-glycerol Mycoplasma genitalium 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
2.4.1.315 UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
-
 Mycoplasma genitalium 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP main product ?
2.4.1.315 UDP-alpha-D-glucose + 1,2-diacyl-sn-glycerol
-
 Mycoplasma genitalium 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.315 MG517
-
 Mycoplasma genitalium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.315 0.004
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.023
-
 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.03
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.27
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 1.1
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 1.13
-
 1,2-diacyl-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.315 0.03
-
 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.063
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 0.08
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 3.1
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 4
-
 1,2-diacyl-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
2.4.1.315 5
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium