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Literature summary extracted from

  • Hargrove, T.Y.; Wawrzak, Z.; Liu, J.; Nes, W.D.; Waterman, M.R.; Lepesheva, G.I.
    Substrate preferences and catalytic parameters determined by structural characteristics of sterol 14alpha-demethylase (CYP51) from Leishmania infantum (2011), J. Biol. Chem., 286, 26838-26848.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expressed in Escherichia coli Leishmania infantum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.154 hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, and 9% (w/v) PEG 6000 Leishmania infantum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 fluconazole the inhibitor not only replaces the substrate in the enzyme active site but most likely displaces the CO from the iron coordination sphere Leishmania infantum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.154 0.00911
-
 14alpha-methylzymosterol at pH 7.4 and 37°C Leishmania infantum
1.14.14.154 0.01131
-
 obtusifoliol at pH 7.4 and 37°C Leishmania infantum
1.14.14.154 0.01204
-
 lanosterol at pH 7.4 and 37°C Leishmania infantum
1.14.14.154 0.01852
-
 norlanosterol at pH 7.4 and 37°C Leishmania infantum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.154 54000
-
 x * 54000, calculated from amino acid sequence Leishmania infantum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.154 14alpha-methylzymosterol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum
-
 ?
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum i.e. lanosta-8,24-dien-3beta-ol 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum i.e. 4alpha,14alpha-dimethylcholesta-8,24-dien-3beta-ol ?
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum i.e. 4alpha,14alpha-dimethyl-5'-ergosta-8,24(24')-dien-3beta-ol 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Leishmania infantum A2TEF2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 14alpha-methylzymosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Leishmania infantum ?
-
 ?
1.14.14.154 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 i.e. anosta-8-en-3ebta-ol Leishmania infantum ?
-
 ?
1.14.14.154 eburicol + [reduced NADPH-hemoprotein reductase] + O2 i.e. 24-methylenelanosta-8-en-3beta-ol Leishmania infantum ?
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 i.e. lanosta-8,24-dien-3beta-ol Leishmania infantum 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 i.e. 4alpha,14alpha-dimethylcholesta-8,24-dien-3beta-ol Leishmania infantum ?
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 i.e. 4alpha,14alpha-dimethyl-5'-ergosta-8,24(24')-dien-3beta-ol Leishmania infantum 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.154 ? x * 54000, calculated from amino acid sequence Leishmania infantum

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
 Leishmania infantum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.154 Leishmania infantum calculated from amino acid sequence
-
 7.7