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Literature summary extracted from

  • Schurmann, K.; Anton, M.; Ivanov, I.; Richter, C.; Kuhn, H.; Walther, M.
    Molecular basis for the reduced catalytic activity of the naturally occurring T560M mutant of human 12/15-lipoxygenase that has been implicated in coronary artery disease (2011), J. Biol. Chem., 286, 23920-23927.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 expressed in Escherichia coli as a His-tagged fusion protein Mus musculus
1.13.11.31 expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens
1.13.11.33 expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.31 Q294L relative catalytic activity: 16.1% compared to wild-type 100% Homo sapiens
1.13.11.31 Q303L mutant shows strongly impared catalytic activity, relative catalytic activity: 3.2% compared to wild-type 100% Mus musculus
1.13.11.31 T560M mutant shows impaired catalytic activity, relative catalytic activity: 25% compared to wild-type 100% Homo sapiens
1.13.11.31 T570M mutant shows strongly impared catalytic activity, relative catalytic activity: 8.2% compared to wild-type 100% Mus musculus
1.13.11.33 N287D relative catalytic activity: 1.1% compared to wild-type 100% Homo sapiens
1.13.11.33 N287L relative catalytic activity: 0.5% compared to wild-type 100% Homo sapiens
1.13.11.33 N287Q relative catalytic activity: 3.9% compared to wild-type 100% Homo sapiens
1.13.11.33 Q294L relative catalytic activity: 0.6% compared to wild-type 100% Homo sapiens
1.13.11.33 T560A mutant shows strongly impaired catalytic activity, relative catalytic activity: 16.6% compared to wild-type 100% Homo sapiens
1.13.11.33 T560M mutant shows strongly impaired catalytic activity, relative catalytic activity: 3.8% compared to wild-type 100% Homo sapiens
1.13.11.33 T560S enzyme retains activity, relative catalytic activity: 70.7% compared to wild-type 100% Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens
-
-
-
1.13.11.31 Mus musculus
-
-
-
1.13.11.33 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 using Ni-NTA chromatography Mus musculus
1.13.11.31 using Ni-NTA chromatography Homo sapiens
1.13.11.33 using Ni-NTA chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonic acid + O2
-
 Mus musculus ?
-
 ?
1.13.11.31 arachidonic acid + O2
-
 Homo sapiens ?
-
 ?
1.13.11.33 arachidonic acid + O2
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 ALOX12
-
 Homo sapiens
1.13.11.31 ALOX5
-
 Mus musculus
1.13.11.33 Alox15
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.31 25
-
 assay at Mus musculus
1.13.11.31 25
-
 assay at Homo sapiens
1.13.11.33 25
-
 assay at Homo sapiens