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Literature summary extracted from

  • Yang, Z.Y.; Dean, D.R.; Seefeldt, L.C.
    Molybdenum nitrogenase catalyzes the reduction and coupling of CO to form hydrocarbons (2011), J. Biol. Chem., 286, 19417-19421.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.18.6.1 synthesis MoFe subunit mutants V70A and V70G will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane. The rates and ratios of hydrocarbon production from CO can be adjusted by changing the flux of electrons through nitrogenase, by substitution of other amino acids located near the FeMo-cofactor, or by changing the partial pressure of CO. Increasing the partial pressure of CO shifts the product ratio in favor of the longer chain alkanes and alkenes Azotobacter vinelandii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.6.1
-
 Azotobacter vinelandii

Protein Variants

EC Number Protein Variants Comment Organism
1.18.6.1 V70A mutation in MoFe subunit. Mutant protein will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane Azotobacter vinelandii
1.18.6.1 V70G mutation in MoFe subunit. Mutant protein will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter vinelandii
-
-
-
1.18.6.1 Azotobacter vinelandii DJ1260
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 CO + dithionite + H+ no substrate for wild-type Azotobacter vinelandii CH4 + C2H6 + C2H4 + C3H6 + C3H8 + ? MoFe subunit mutants V70A and V70G will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane. The rates and ratios of hydrocarbon production from CO can be adjusted by changing the flux of electrons through nitrogenase, by substitution of other amino acids located near the FeMo-cofactor, or by changing the partial pressure of CO. Increasing the partial pressure of CO shifts the product ratio in favor of the longer chain alkanes and alkenes ?
1.18.6.1 CO + dithionite + H+ no substrate for wild-type Azotobacter vinelandii DJ1260 CH4 + C2H6 + C2H4 + C3H6 + C3H8 + ? MoFe subunit mutants V70A and V70G will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane. The rates and ratios of hydrocarbon production from CO can be adjusted by changing the flux of electrons through nitrogenase, by substitution of other amino acids located near the FeMo-cofactor, or by changing the partial pressure of CO. Increasing the partial pressure of CO shifts the product ratio in favor of the longer chain alkanes and alkenes ?