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Literature summary extracted from

  • Klaus, S.M.; Wegkamp, A.; Sybesma, W.; Hugenholtz, J.; Gregory, J.F. 3rd, Hanson, A.D.
    A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants (2004), J. Biol. Chem., 280, 5274-5280.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.67 expression in Escherichia coli Lactococcus lactis
3.6.1.67 expression in Escherichia coli Arabidopsis thaliana
3.6.1.67 expression in Escherichia coli with and without a C-terminal His tag Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.67 0.0021
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Lactococcus lactis
3.6.1.67 0.0021
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Arabidopsis thaliana
3.6.1.67 0.147
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.67 Mg2+ required, no activity with Ca2+ Arabidopsis thaliana
3.6.1.67 Mg2+ requires a physiological level (approximately 1 mM) of Mg2+ Lactococcus lactis
3.6.1.67 Mg2+ the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+ Lactococcus lactis
3.6.1.67 Mg2+ the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.67 20400
-
 1 * 20400, calculated from sequence Lactococcus lactis
3.6.1.67 20400
-
 1 * 20400, calculated from sequence Arabidopsis thaliana
3.6.1.67 24200
-
 gel filtration Lactococcus lactis
3.6.1.67 24200
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O Lactococcus lactis the second step in the pterin branch of the folate synthesis pathway 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O Lactococcus lactis NZ9000 the second step in the pterin branch of the folate synthesis pathway 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.67 Arabidopsis thaliana Q9CA40
-
-
3.6.1.67 Lactococcus lactis D8KIT5
-
-
3.6.1.67 Lactococcus lactis NZ9000 D8KIT5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.67
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.67 0.0017
-
 substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30°C Arabidopsis thaliana
3.6.1.67 0.65
-
 substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30°C Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O the second step in the pterin branch of the folate synthesis pathway Lactococcus lactis 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O no activity against deoxynucleoside triphosphates Lactococcus lactis 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O the enzyme is far more active against dGTP, 8-oxo-GTP, dTTP, and other nucleoside and deoxynucleoside 3'-triphosphates than against 7,8-dihydroneopterin 3'-triphosphate Arabidopsis thaliana 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O catalytic efficiency of the enzyme is far higher for dGTP Arabidopsis thaliana 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate) Lactococcus lactis 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O the second step in the pterin branch of the folate synthesis pathway Lactococcus lactis NZ9000 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O no activity against deoxynucleoside triphosphates Lactococcus lactis NZ9000 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?
3.6.1.67 7,8-dihydroneopterin 3'-triphosphate + H2O no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate) Lactococcus lactis NZ9000 7,8-dihydroneopterin 3'-phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.67 monomer 1 * 20400, calculated from sequence Lactococcus lactis
3.6.1.67 monomer 1 * 20400, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.6.1.67 At1g68760
-
 Arabidopsis thaliana
3.6.1.67 At1g68760 protein
-
 Arabidopsis thaliana
3.6.1.67 AtNUDT1
-
 Arabidopsis thaliana
3.6.1.67 DHNTP pyrophosphohydrolase
-
 Lactococcus lactis
3.6.1.67 DHNTP pyrophosphohydrolase
-
 Arabidopsis thaliana
3.6.1.67 dihydroneopterin triphosphate pyrophosphohydrolase
-
 Lactococcus lactis
3.6.1.67 dihydroneopterin triphosphate pyrophosphohydrolase
-
 Arabidopsis thaliana
3.6.1.67 folQ
-
 Lactococcus lactis
3.6.1.67 YlgG
-
 Lactococcus lactis
3.6.1.67 YlgG former gene name Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.67 30
-
 assay at Lactococcus lactis
3.6.1.67 30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.67 0.0006
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Arabidopsis thaliana
3.6.1.67 0.21
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Lactococcus lactis
3.6.1.67 0.21
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.67 8
-
 assay at Lactococcus lactis
3.6.1.67 8
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.6.1.67 physiological function the enzyme functions in folate biosynthesis Lactococcus lactis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.67 0.0041
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Arabidopsis thaliana
3.6.1.67 100
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Lactococcus lactis
3.6.1.67 100
-
 7,8-dihydroneopterin 3'-triphosphate pH 8.0, 30°C Arabidopsis thaliana