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Literature summary extracted from

  • Miles, R.D.; Gorrell, A.; Ferry, J.G.
    Evidence for a transition state analog, MgADP-aluminum fluoride-acetate, in acetate kinase from Methanosarcina thermophila (2002), J. Biol. Chem., 277, 22547-22552.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 recombinant acetate kinase with a 6-residue N-terminal histidine tag is heterologously produced in Escherichia coli BL21(DE3) Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 acetate inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition) Methanosarcina thermophila
2.7.2.1 ADP inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site Methanosarcina thermophila
2.7.2.1 AlCl3 inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Protection from inhibition by a non-hydrolyzable ATP analog or acetylphosphate. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
2.7.2.1 ATP-gamma-S non-hydrolyzable inhibitor Methanosarcina thermophila
2.7.2.1 CDP preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
2.7.2.1 IDP preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
2.7.2.1 MgCl2 inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
2.7.2.1 additional information preincubation with butyrate does not significantly inhibit the enzyme Methanosarcina thermophila
2.7.2.1 NaF inhibition by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate. When MgCl2, ADP, and acetate are omitted from the preincubation mixture, there is no detectable loss of activity; inhibition of acetate kinase by preincubation with MgCl2, ADP, AlCl3, NaF, and acetate (all of the components are necessary for maximum inhibition). The transition state analog, MgADP-aluminum fluoride-acetate, forms an abortive complex in the active site. Preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila
2.7.2.1 propionate preincubation with MgCl2, ADP, AlCl3, NaF, and propionate results in almost complete inhibition of activity Methanosarcina thermophila
2.7.2.1 UDP preincubation of acetate kinase with MgCl2, AlCl3, NaF, acetate, and either IDP, UDP, or CDP in place of ADP results in almost complete inhibition of activity Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila P38502
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 5.5
-
 assay at Methanosarcina thermophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.1 0.0184
-
 ADP pH and temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 0.95
-
 AlCl3 pH and temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 0.95
-
 MgCl2 pH 5.5, temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 2.9
-
 acetate pH and temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 2.9
-
 acetate pH 5.5, temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 3
-
 NaF pH and temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 3.4
-
 MgCl2 pH and temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 3.4
-
 AlCl3 pH 5.5, temperature not specified in the publication Methanosarcina thermophila
2.7.2.1 18.4
-
 ADP pH 5.5, temperature not specified in the publication Methanosarcina thermophila