BRENDA - Enzyme Database

Comparison of isocitrate dehydrogenase from three hyperthermophiles reveals differences in thermostability, cofactor specificity, oligomeric state, and phylogenetic affiliation

Steen, I.H.; Madern, D.; Karlstrm, M.; Lien, T.; Ladenstein, R.; Birkeland, N.K.; J. Biol. Chem. 276, 43924-43931 (2001)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.0683
-
NAD+
pH 8.0, 70C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+
Pyrococcus furiosus
1.1.1.42
0.0444
-
NADP+
pH 8.0, 70C
Aeropyrum pernix
1.1.1.42
0.0552
-
NADP+
pH 8.0, 70C, Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
Thermotoga maritima
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
44300
-
2 * 44300, calculated from sequence
Pyrococcus furiosus
1.1.1.41
70000
-
analytical ultracentrifugation
Pyrococcus furiosus
1.1.1.42
45400
-
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence; 4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence
Thermotoga maritima
1.1.1.42
47900
-
2 * 47900, calculated from sequence
Aeropyrum pernix
1.1.1.42
68000
-
dimer, analytical ultracentrifugation
Thermotoga maritima
1.1.1.42
70000
-
analytical ultracentrifugation
Aeropyrum pernix
1.1.1.42
174000
-
tetramer, analytical ultracentrifugation
Thermotoga maritima
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermotoga maritima
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Aeropyrum pernix
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Aeropyrum pernix DSM 11879
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Thermotoga maritima DSM 3109
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Pyrococcus furiosus
Q8U488
-
-
1.1.1.42
Aeropyrum pernix
Q9YE81
-
-
1.1.1.42
Aeropyrum pernix DSM 11879
Q9YE81
-
-
1.1.1.42
Thermotoga maritima
Q9X0N2
-
-
1.1.1.42
Thermotoga maritima DSM 3109
Q9X0N2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
-
Pyrococcus furiosus
1.1.1.42
-
Aeropyrum pernix
1.1.1.42
-
Thermotoga maritima
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
low activity with NADP+. Km-value for NADP+ is about 65fold higher than the KM-value for NAD+
727835
Pyrococcus furiosus
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Thermotoga maritima
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Aeropyrum pernix
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
727835
Thermotoga maritima
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Aeropyrum pernix DSM 11879
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Thermotoga maritima DSM 3109
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
727835
Thermotoga maritima DSM 3109
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
homodimer
2 * 44300, calculated from sequence
Pyrococcus furiosus
1.1.1.42
dimer
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence
Thermotoga maritima
1.1.1.42
homodimer
2 * 47900, calculated from sequence
Aeropyrum pernix
1.1.1.42
tetramer
4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence
Thermotoga maritima
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.41
95
-
above
Pyrococcus furiosus
1.1.1.42
90
-
-
Thermotoga maritima
1.1.1.42
95
-
above
Aeropyrum pernix
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.41
additional information
-
thermal denaturation is an irreversible process
Pyrococcus furiosus
1.1.1.41
104
-
the Tm-value is 103.7C
Pyrococcus furiosus
1.1.1.42
additional information
-
thermal denaturation is an irreversible process
Aeropyrum pernix
1.1.1.42
additional information
-
thermal denaturation is an irreversible process
Thermotoga maritima
1.1.1.42
98
-
the Tm-value is 98.3C
Thermotoga maritima
1.1.1.42
110
-
the Tm-value is 109.9C
Aeropyrum pernix
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.0683
-
NAD+
pH 8.0, 70C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+
Pyrococcus furiosus
1.1.1.42
0.0444
-
NADP+
pH 8.0, 70C
Aeropyrum pernix
1.1.1.42
0.0552
-
NADP+
pH 8.0, 70C, Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
Thermotoga maritima
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
44300
-
2 * 44300, calculated from sequence
Pyrococcus furiosus
1.1.1.41
70000
-
analytical ultracentrifugation
Pyrococcus furiosus
1.1.1.42
45400
-
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence; 4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence
Thermotoga maritima
1.1.1.42
47900
-
2 * 47900, calculated from sequence
Aeropyrum pernix
1.1.1.42
68000
-
dimer, analytical ultracentrifugation
Thermotoga maritima
1.1.1.42
70000
-
analytical ultracentrifugation
Aeropyrum pernix
1.1.1.42
174000
-
tetramer, analytical ultracentrifugation
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermotoga maritima
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Aeropyrum pernix
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Aeropyrum pernix DSM 11879
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
isocitrate + NADP+
Thermotoga maritima DSM 3109
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
-
Pyrococcus furiosus
1.1.1.42
-
Aeropyrum pernix
1.1.1.42
-
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
low activity with NADP+. Km-value for NADP+ is about 65fold higher than the KM-value for NAD+
727835
Pyrococcus furiosus
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Thermotoga maritima
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Aeropyrum pernix
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
727835
Thermotoga maritima
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Aeropyrum pernix DSM 11879
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
727835
Thermotoga maritima DSM 3109
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
isocitrate + NADP+
low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
727835
Thermotoga maritima DSM 3109
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
homodimer
2 * 44300, calculated from sequence
Pyrococcus furiosus
1.1.1.42
dimer
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence
Thermotoga maritima
1.1.1.42
homodimer
2 * 47900, calculated from sequence
Aeropyrum pernix
1.1.1.42
tetramer
4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence
Thermotoga maritima
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.41
95
-
above
Pyrococcus furiosus
1.1.1.42
90
-
-
Thermotoga maritima
1.1.1.42
95
-
above
Aeropyrum pernix
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.41
additional information
-
thermal denaturation is an irreversible process
Pyrococcus furiosus
1.1.1.41
104
-
the Tm-value is 103.7C
Pyrococcus furiosus
1.1.1.42
additional information
-
thermal denaturation is an irreversible process
Aeropyrum pernix
1.1.1.42
additional information
-
thermal denaturation is an irreversible process
Thermotoga maritima
1.1.1.42
98
-
the Tm-value is 98.3C
Thermotoga maritima
1.1.1.42
110
-
the Tm-value is 109.9C
Aeropyrum pernix