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Literature summary extracted from

  • Kadrmas, J.L.; Raetz, C.R.
    Enzymatic synthesis of lipopolysaccharide in Escherichia coli. Purification and properties of heptosyltransferase I (1998), J. Biol. Chem., 273, 2799-2807.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.99.23 Triton X-100 the enzymatic activity is dependent upon the presence of Triton X-100. At concentrations greater than 0.1% Triton X-100, the transferase activity is inhibited Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.23
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.23 Triton X-100 the enzymatic activity is dependent upon the presence of Triton X-100. At concentrations greater than 0.1% Triton X-100, the transferase activity is inhibited Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.99.23 0.0045
-
 (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose pH 7.5, 30°C Escherichia coli
2.4.99.23 1.5
-
 ADP-alpha-D-mannose pH 7.5, 30°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.99.23 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.23 36000
-
 x * 36000, gel electrophoresis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.23
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.99.23 4.9
-
 pH 7.5, 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.23 ADP-alpha-D-mannose + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose i.e. alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA. In cell extracts, ADP-mannose can serve as an alternative donor, but is mannosylated at less than 1% the rate of alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA Escherichia coli ADP + ?
-
 ?
2.4.99.23 ADP-L-glycero-beta-D-manno-heptose + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose i.e. alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA. It is possible to assay the enzyme in crude cell extracts using ADP-mannose in place of ADP-L-glycero-beta-D-manno-heptose. No activity without detergent Escherichia coli ADP + alpha-L-glycero-D-manno-heptosyl-(1->5)-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose
-
 ?
2.4.99.23 additional information the purified enzyme displays no activity with ADP-glucose, GDP-mannose, UDP-glucose, or UDP-galactose Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.99.23 ? x * 36000, gel electrophoresis Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.99.23 heptosyltransferase I
-
 Escherichia coli
2.4.99.23 rfaC
-
 Escherichia coli
2.4.99.23 waaC
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.99.23 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.23 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.99.23 7.2 8 pH 7.2: about 70% of maximal activity, pH 8.0: about 55% of maximal activity Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.99.23 malfunction lipopolysaccharide isolated from mutants defective in rfaC lack heptose and all other sugars distal to heptose Escherichia coli