Literature summary extracted from

Carlson, K.; Krabbe, M.; Nystroem, A.C.; Kosturko, L.D.
DNA determinants of restriction. Bacteriophage T4 endonuclease II-dependent cleavage of plasmid DNA in vivo (1993), J. Biol. Chem., 268, 8908-8918.

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.21.8	Tequatrovirus T4	P07059	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.21.8	plasmid pBR322 + H2O	-	Tequatrovirus T4	?	double-stranded restriction cleavage at 12 sites in pBR322 commence before 10-min postinfection with T4 at 37°C and proceeds more slowly in the presence of competing phage DNA than in its absence, utilizing the same sites in both cases. In a 200-base pair segment of the plasmid, single-stranded nicks also are frequent. The plasmid sites are cleaved with a speed that varies with the site, yielding frequencies of cleavage at different sites varying between 10 and 90%, at 50-min postinfection. All sites contain good matches to a consensus, 5'-GRCCGCNTYGC-3', most frequently cleaved around the variable central base pair, generating fragments with blunt ends or 1-2-base 5' overhangs. A larger consensus sequence, 5'-CGRCCGCNTTGSYNGC-3', has been identified. DNA sequence elements 3' to the cut site appear important for rapid cleavage	?

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Release 2023.1 (January 2023)