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Literature summary extracted from

  • Bafana, A.; Chakrabarti, T.; Krishnamurthi, K.
    Mercuric reductase activity of multiple heavy metal-resistant Lysinibacillus sphaericus G1 (2015), J. Basic Microbiol., 55, 285-992.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.1.1 gene merA, DNA and amino acid sequence determination and analysis, pylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+ Lysinibacillus sphaericus
-
 Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+ Lysinibacillus sphaericus G1
-
 Hg2+ + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.1 Lysinibacillus sphaericus D9J041 i.e. Bacillus sphaericus, isolated from an industrial mercuric salt-contaminated soil, gene merA
-
1.16.1.1 Lysinibacillus sphaericus G1 D9J041 i.e. Bacillus sphaericus, isolated from an industrial mercuric salt-contaminated soil, gene merA
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.1.1 additional information growth of strain G1 at sublethal concentrations of metal salts, Cd2+, Zn2+, Co2+, and K2Cr2O7-, in presence of absence of Hg2+, overview Lysinibacillus sphaericus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16.1.1 0.13
-
 cell extract, mercury-dependent oxidation of NADPH, pH 7.4, 25°C Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+
-
 Lysinibacillus sphaericus Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+
-
 Lysinibacillus sphaericus G1 Hg2+ + NADPH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.16.1.1 MerA
-
 Lysinibacillus sphaericus
1.16.1.1 mercuric reductase
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.16.1.1 25
-
 assay at Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.16.1.1 7.4
-
 assay at Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.1 NADP+
-
 Lysinibacillus sphaericus
1.16.1.1 NADPH
-
 Lysinibacillus sphaericus