BRENDA - Enzyme Database

Regulatory and functional diversity of methylmercaptopropionate coenzyme A ligases from the dimethylsulfoniopropionate demethylation pathway in Ruegeria pomeroyi DSS-3 and other proteobacteria

Bullock, H.A.; Reisch, C.R.; Burns, A.S.; Moran, M.A.; Whitman, W.B.; J. Bacteriol. 196, 1275-1285 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.2.1.44
expression in Escherichia coli
Burkholderia thailandensis
6.2.1.44
expression in Escherichia coli
Candidatus Pelagibacter ubique
6.2.1.44
expression in Escherichia coli
Pseudomonas aeruginosa
6.2.1.44
expression in Escherichia coli; expression in Escherichia coli
Ruegeria lacuscaerulensis
6.2.1.44
expression in Escherichia coli; expression in Escherichia coli
Ruegeria pomeroyi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.44
(NH4)2SO4
0.4 M, 93% inhibition
Burkholderia thailandensis
6.2.1.44
(NH4)2SO4
0.4 M, 14% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
(NH4)2SO4
0.4 M, 29% inhibition; 0.4 M, 43% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
(NH4)2SO4
0.4 M, 75% inhibition
Ruegeria pomeroyi
6.2.1.44
dimethylsulfoniopropionate
inhibited by physiological concentrations (70 mM). ADP reverses the inhibition of RPO_DmdB1, suggesting that this enzyme was responsive to cellular energy charge; inhibited by physiological concentrations (70 mM). ADP reverses the inhibition of RPO_DmdB1, suggesting that this enzyme was responsive to cellular energy charge. 3-(Methylthio)propanoate reverses the inhibition of RPO_DmdB2, suggesting that a complex regulatory system exists
Ruegeria pomeroyi
6.2.1.44
K2SO4
0.4 M, 16% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
K2SO4
0.4 M, 5% inhibition; 0.4 M, 90% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
K2SO4
0.4 M, 68% inhibition
Ruegeria pomeroyi
6.2.1.44
KCl
0.4 M, 73% inhibition
Burkholderia thailandensis
6.2.1.44
KCl
0.4 M, 8% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
KCl
0.4 M, 55% inhibition; 0.4 M, 70% inhibition
Ruegeria pomeroyi
6.2.1.44
Li2SO4
0.4 M, 92% inhibition
Burkholderia thailandensis
6.2.1.44
Li2SO4
0.4 M, 92% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
Li2SO4
0.4 M, 41% inhibition; 0.4 M, 57% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Li2SO4
0.4 M, 82% inhibition; 0.4 M, 82% inhibition
Ruegeria pomeroyi
6.2.1.44
LiCl
0.4 M, 87% inhibition
Burkholderia thailandensis
6.2.1.44
LiCl
0.4 M, 91% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
LiCl
0.4 M, 59% inhibition
Ruegeria pomeroyi
6.2.1.44
additional information
no inhibition by dimethylsulfoniopropionate
Burkholderia thailandensis
6.2.1.44
additional information
no inhibition by dimethylsulfoniopropionate
Pseudomonas aeruginosa
6.2.1.44
additional information
no inhibition by dimethylsulfoniopropionate; no inhibition by dimethylsulfoniopropionate
Ruegeria lacuscaerulensis
6.2.1.44
Na2SO4
0.4 M, 84% inhibition
Burkholderia thailandensis
6.2.1.44
Na2SO4
0.4 M, 60% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
Na2SO4
0.4 M, 58% inhibition; 0.4 M, 66% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Na2SO4
0.4 M, 68% inhibition
Ruegeria pomeroyi
6.2.1.44
NaCl
0.4 M, 92% inhibition
Burkholderia thailandensis
6.2.1.44
NaCl
0.4 M, 57% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
NaCl
0.4 M,10% inhibition; 0.4 M, 14% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
NaCl
0.4 M, 50% inhibition; 0.4 M, 60% inhibition
Ruegeria pomeroyi
6.2.1.44
NH4Cl
0.4 M, 55% inhibition
Burkholderia thailandensis
6.2.1.44
NH4Cl
0.4 M, 32% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
NH4Cl
0.4 M, 50% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
NH4Cl
0.4 M, 94% inhibition
Ruegeria pomeroyi
6.2.1.44
potassium acetate
0.4 M, 7% inhibition
Burkholderia thailandensis
6.2.1.44
potassium acetate
0.4 M, 16% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
potassium acetate
0.4 M, 56% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
potassium acetate
0.4 M, 51% inhibition; 0.4 M, 73% inhibition
Ruegeria pomeroyi
6.2.1.44
Sodium acetate
0.4 M, 45% inhibition
Burkholderia thailandensis
6.2.1.44
Sodium acetate
0.4 M, 61% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
Sodium acetate
0.4 M, 68% inhibition; 0.4 M, 88% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Sodium acetate
0.4 M, 66% inhibition; 0.4 M, 86% inhibition
Ruegeria pomeroyi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.44
0.01
-
Butyrate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.02
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.04
-
3-(methylthio)propanoate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.04
-
propionate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.04
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.07
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.08
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.1
2
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.44
-
Acrylate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.9
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
3.11
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
5.25
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.44
(NH4)2SO4
0.4 M, 1.5fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
K2SO4
0.4 M, 1.3fold enhancement of activity
Burkholderia thailandensis
6.2.1.44
K2SO4
0.4 M, 1.6fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
KCl
0.4 M, 1.4fold enhancement of activity
Ruegeria lacuscaerulensis
6.2.1.44
LiCl
0.4 M, 1.4fold enhancement of activity; 0.4 M, 1.4fold enhancement of activity
Ruegeria lacuscaerulensis
6.2.1.44
LiCl
0.4 M, 3fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
Mg2+
required for activity
Burkholderia thailandensis
6.2.1.44
Mg2+
required for activity
Candidatus Pelagibacter ubique
6.2.1.44
Mg2+
required for activity
Pseudomonas aeruginosa
6.2.1.44
Mg2+
required for activity; required for activity
Ruegeria lacuscaerulensis
6.2.1.44
Mg2+
required for activity; required for activity
Ruegeria pomeroyi
6.2.1.44
Na2SO4
0.4 M, 1.2fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
NH4Cl
0.4 M, 1.6fold enhancement of activity
Ruegeria lacuscaerulensis
6.2.1.44
NH4Cl
0.4 M, 2.1fold enhancement of activity
Ruegeria pomeroyi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.44
58000
-
2 * 58000, SDS-PAGE
Ruegeria pomeroyi
6.2.1.44
59000
-
2 * 59000, SDS-PAGE
Candidatus Pelagibacter ubique
6.2.1.44
59066
-
2 * 59066, calculated from sequence
Ruegeria pomeroyi
6.2.1.44
59143
-
x * 59143, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
59166
-
2 * 59166, calculated from sequence
Ruegeria pomeroyi
6.2.1.44
59400
-
x * 59400, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
59548
-
x * 59548, calculated from sequence
Pseudomonas aeruginosa
6.2.1.44
61340
-
2 * 61340, calculated from sequence
Candidatus Pelagibacter ubique
6.2.1.44
62000
-
2 * 62000, SDS-PAGE
Ruegeria pomeroyi
6.2.1.44
64781
-
x * 64781, calculated from sequence
Burkholderia thailandensis
6.2.1.44
108000
-
gel filtration
Candidatus Pelagibacter ubique
6.2.1.44
112000
-
gel filtration
Ruegeria pomeroyi
6.2.1.44
122000
-
gel filtration
Ruegeria pomeroyi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
Candidatus Pelagibacter ubique
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
Ruegeria pomeroyi
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
Ruegeria pomeroyi
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
AMP + diphosphate + 3-methylmercaptopropionyl-CoA
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.44
Burkholderia thailandensis
Q2SWN7
-
-
6.2.1.44
Burkholderia thailandensis ATCC 700388
Q2SWN7
-
-
6.2.1.44
Candidatus Pelagibacter ubique
Q4FP19
-
-
6.2.1.44
Candidatus Pelagibacter ubique HTCC1062
Q4FP19
-
-
6.2.1.44
Pseudomonas aeruginosa
Q9HWI3
-
-
6.2.1.44
Pseudomonas aeruginosa ATCC 15692
Q9HWI3
-
-
6.2.1.44
Ruegeria lacuscaerulensis
D0CPY8
-
-
6.2.1.44
Ruegeria lacuscaerulensis
D0CV95
-
-
6.2.1.44
Ruegeria lacuscaerulensis ITI-1157
D0CPY8
-
-
6.2.1.44
Ruegeria lacuscaerulensis ITI-1157
D0CV95
-
-
6.2.1.44
Ruegeria pomeroyi
Q5LRT0
-
-
6.2.1.44
Ruegeria pomeroyi
Q5LVM3
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.44
-
Burkholderia thailandensis
6.2.1.44
-
Candidatus Pelagibacter ubique
6.2.1.44
-
Pseudomonas aeruginosa
6.2.1.44
;
Ruegeria lacuscaerulensis
6.2.1.44
;
Ruegeria pomeroyi
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.2.1.44
15
-
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
16
-
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
28
-
pH 7.5, 22°C
Candidatus Pelagibacter ubique
Storage Stability
EC Number
Storage Stability
Organism
6.2.1.44
-20°C, stable in 100 mM HEPES for up to 6 months
Candidatus Pelagibacter ubique
6.2.1.44
-20°C, stable in a solution of 100 mM KHPO4 for up to 2 months
Ruegeria pomeroyi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Pseudomonas aeruginosa
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Burkholderia thailandensis
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-methylmercaptopropionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 14% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 15% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 18% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 19% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 4% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 5% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 8% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 110% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 151% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 110% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 151% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 15% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 29% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 50% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 56% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 6% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 65% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 78% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 65% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 50% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 15% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 29% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 6% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 113% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 121% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 160% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 36% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 73% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 81% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 93% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 121% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 160% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 93% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 113% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 81% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 109% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 2% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 32% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 41% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 63% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 63% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 87% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 12% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 17% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 17% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 27% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 5% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 62% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 94% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 12% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 13% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 30% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 48% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 5% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 7% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 18% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 20% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 23% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 24% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 39% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 8% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 179% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 18% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 31% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 49% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 51% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 68% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 91% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 68% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 179% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 124% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 126% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 13% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 16% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 45% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 72% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 77% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 124% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 13% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 45% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 72% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 126% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Ruegeria pomeroyi
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Ruegeria lacuscaerulensis
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Burkholderia thailandensis
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Ruegeria pomeroyi
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Candidatus Pelagibacter ubique
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Ruegeria lacuscaerulensis
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Pseudomonas aeruginosa
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Pseudomonas aeruginosa ATCC 15692
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Candidatus Pelagibacter ubique HTCC1062
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Burkholderia thailandensis ATCC 700388
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Ruegeria lacuscaerulensis ITI-1157
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Ruegeria lacuscaerulensis ITI-1157
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.2.1.44
?
x * 64781, calculated from sequence
Burkholderia thailandensis
6.2.1.44
?
x * 59548, calculated from sequence
Pseudomonas aeruginosa
6.2.1.44
?
x * 59143, calculated from sequence; x * 59400, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
dimer
2 * 59000, SDS-PAGE; 2 * 61340, calculated from sequence
Candidatus Pelagibacter ubique
6.2.1.44
dimer
2 * 58000, SDS-PAGE; 2 * 59066, calculated from sequence; 2 * 59166, calculated from sequence; 2 * 62000, SDS-PAGE
Ruegeria pomeroyi
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.2.1.44
22
-
assay at room temperature
Candidatus Pelagibacter ubique
6.2.1.44
22
-
assay at room temperature; assay at room temperature
Ruegeria pomeroyi
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.2.1.44
10
30
activity at 10°C and at 30°C is nearly the same as at room temperature
Candidatus Pelagibacter ubique
6.2.1.44
10
30
activity at 10°C and at 30°C is nearly the same as at room temperature; activity at 10°C and at 30°C is nearly the same as at room temperature
Ruegeria pomeroyi
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.2.1.44
1
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
3.7
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
7.2
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
10.8
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
14.3
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
14.4
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
14.9
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
17.7
-
propionate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
18.7
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
22.3
-
Acrylate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
29.4
-
3-(methylthio)propanoate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
43.8
-
Butyrate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.44
7
-
-
Ruegeria pomeroyi
6.2.1.44
7.5
-
-
Candidatus Pelagibacter ubique
6.2.1.44
7.5
-
-
Ruegeria pomeroyi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.44
expression in Escherichia coli
Burkholderia thailandensis
6.2.1.44
expression in Escherichia coli
Candidatus Pelagibacter ubique
6.2.1.44
expression in Escherichia coli
Pseudomonas aeruginosa
6.2.1.44
expression in Escherichia coli
Ruegeria lacuscaerulensis
6.2.1.44
expression in Escherichia coli
Ruegeria pomeroyi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.44
(NH4)2SO4
0.4 M, 93% inhibition
Burkholderia thailandensis
6.2.1.44
(NH4)2SO4
0.4 M, 14% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
(NH4)2SO4
0.4 M, 43% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
(NH4)2SO4
0.4 M, 29% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
(NH4)2SO4
0.4 M, 75% inhibition
Ruegeria pomeroyi
6.2.1.44
dimethylsulfoniopropionate
inhibited by physiological concentrations (70 mM). ADP reverses the inhibition of RPO_DmdB1, suggesting that this enzyme was responsive to cellular energy charge. 3-(Methylthio)propanoate reverses the inhibition of RPO_DmdB2, suggesting that a complex regulatory system exists
Ruegeria pomeroyi
6.2.1.44
dimethylsulfoniopropionate
inhibited by physiological concentrations (70 mM). ADP reverses the inhibition of RPO_DmdB1, suggesting that this enzyme was responsive to cellular energy charge
Ruegeria pomeroyi
6.2.1.44
K2SO4
0.4 M, 16% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
K2SO4
0.4 M, 5% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
K2SO4
0.4 M, 90% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
K2SO4
0.4 M, 68% inhibition
Ruegeria pomeroyi
6.2.1.44
KCl
0.4 M, 73% inhibition
Burkholderia thailandensis
6.2.1.44
KCl
0.4 M, 8% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
KCl
0.4 M, 55% inhibition
Ruegeria pomeroyi
6.2.1.44
KCl
0.4 M, 70% inhibition
Ruegeria pomeroyi
6.2.1.44
Li2SO4
0.4 M, 92% inhibition
Burkholderia thailandensis
6.2.1.44
Li2SO4
0.4 M, 92% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
Li2SO4
0.4 M, 57% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Li2SO4
0.4 M, 41% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Li2SO4
0.4 M, 82% inhibition
Ruegeria pomeroyi
6.2.1.44
LiCl
0.4 M, 87% inhibition
Burkholderia thailandensis
6.2.1.44
LiCl
0.4 M, 91% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
LiCl
0.4 M, 59% inhibition
Ruegeria pomeroyi
6.2.1.44
additional information
no inhibition by dimethylsulfoniopropionate
Burkholderia thailandensis
6.2.1.44
additional information
no inhibition by dimethylsulfoniopropionate
Pseudomonas aeruginosa
6.2.1.44
additional information
no inhibition by dimethylsulfoniopropionate
Ruegeria lacuscaerulensis
6.2.1.44
Na2SO4
0.4 M, 84% inhibition
Burkholderia thailandensis
6.2.1.44
Na2SO4
0.4 M, 60% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
Na2SO4
0.4 M, 58% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Na2SO4
0.4 M, 66% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Na2SO4
0.4 M, 68% inhibition
Ruegeria pomeroyi
6.2.1.44
NaCl
0.4 M, 92% inhibition
Burkholderia thailandensis
6.2.1.44
NaCl
0.4 M, 57% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
NaCl
0.4 M, 14% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
NaCl
0.4 M,10% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
NaCl
0.4 M, 50% inhibition
Ruegeria pomeroyi
6.2.1.44
NaCl
0.4 M, 60% inhibition
Ruegeria pomeroyi
6.2.1.44
NH4Cl
0.4 M, 55% inhibition
Burkholderia thailandensis
6.2.1.44
NH4Cl
0.4 M, 32% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
NH4Cl
0.4 M, 50% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
NH4Cl
0.4 M, 94% inhibition
Ruegeria pomeroyi
6.2.1.44
potassium acetate
0.4 M, 7% inhibition
Burkholderia thailandensis
6.2.1.44
potassium acetate
0.4 M, 16% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
potassium acetate
0.4 M, 56% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
potassium acetate
0.4 M, 51% inhibition
Ruegeria pomeroyi
6.2.1.44
potassium acetate
0.4 M, 73% inhibition
Ruegeria pomeroyi
6.2.1.44
Sodium acetate
0.4 M, 45% inhibition
Burkholderia thailandensis
6.2.1.44
Sodium acetate
0.4 M, 61% inhibition
Candidatus Pelagibacter ubique
6.2.1.44
Sodium acetate
0.4 M, 68% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Sodium acetate
0.4 M, 88% inhibition
Ruegeria lacuscaerulensis
6.2.1.44
Sodium acetate
0.4 M, 66% inhibition
Ruegeria pomeroyi
6.2.1.44
Sodium acetate
0.4 M, 86% inhibition
Ruegeria pomeroyi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.44
0.01
-
Butyrate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.02
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.04
-
3-(methylthio)propanoate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.04
-
propionate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.04
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.07
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.08
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.1
2
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
0.44
-
Acrylate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
0.9
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
3.11
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
5.25
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.44
(NH4)2SO4
0.4 M, 1.5fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
K2SO4
0.4 M, 1.3fold enhancement of activity
Burkholderia thailandensis
6.2.1.44
K2SO4
0.4 M, 1.6fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
KCl
0.4 M, 1.4fold enhancement of activity
Ruegeria lacuscaerulensis
6.2.1.44
LiCl
0.4 M, 1.4fold enhancement of activity
Ruegeria lacuscaerulensis
6.2.1.44
LiCl
0.4 M, 3fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
Mg2+
required for activity
Burkholderia thailandensis
6.2.1.44
Mg2+
required for activity
Candidatus Pelagibacter ubique
6.2.1.44
Mg2+
required for activity
Pseudomonas aeruginosa
6.2.1.44
Mg2+
required for activity
Ruegeria lacuscaerulensis
6.2.1.44
Mg2+
required for activity
Ruegeria pomeroyi
6.2.1.44
Na2SO4
0.4 M, 1.2fold enhancement of activity
Ruegeria pomeroyi
6.2.1.44
NH4Cl
0.4 M, 1.6fold enhancement of activity
Ruegeria lacuscaerulensis
6.2.1.44
NH4Cl
0.4 M, 2.1fold enhancement of activity
Ruegeria pomeroyi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.2.1.44
58000
-
2 * 58000, SDS-PAGE
Ruegeria pomeroyi
6.2.1.44
59000
-
2 * 59000, SDS-PAGE
Candidatus Pelagibacter ubique
6.2.1.44
59066
-
2 * 59066, calculated from sequence
Ruegeria pomeroyi
6.2.1.44
59143
-
x * 59143, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
59166
-
2 * 59166, calculated from sequence
Ruegeria pomeroyi
6.2.1.44
59400
-
x * 59400, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
59548
-
x * 59548, calculated from sequence
Pseudomonas aeruginosa
6.2.1.44
61340
-
2 * 61340, calculated from sequence
Candidatus Pelagibacter ubique
6.2.1.44
62000
-
2 * 62000, SDS-PAGE
Ruegeria pomeroyi
6.2.1.44
64781
-
x * 64781, calculated from sequence
Burkholderia thailandensis
6.2.1.44
108000
-
gel filtration
Candidatus Pelagibacter ubique
6.2.1.44
112000
-
gel filtration
Ruegeria pomeroyi
6.2.1.44
122000
-
gel filtration
Ruegeria pomeroyi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
Candidatus Pelagibacter ubique
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
Ruegeria pomeroyi
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
Ruegeria pomeroyi
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
AMP + diphosphate + 3-methylmercaptopropionyl-CoA
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.44
-
Burkholderia thailandensis
6.2.1.44
-
Candidatus Pelagibacter ubique
6.2.1.44
-
Pseudomonas aeruginosa
6.2.1.44
-
Ruegeria lacuscaerulensis
6.2.1.44
-
Ruegeria pomeroyi
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.2.1.44
15
-
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
16
-
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
28
-
pH 7.5, 22°C
Candidatus Pelagibacter ubique
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.2.1.44
-20°C, stable in 100 mM HEPES for up to 6 months
Candidatus Pelagibacter ubique
6.2.1.44
-20°C, stable in a solution of 100 mM KHPO4 for up to 2 months
Ruegeria pomeroyi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Pseudomonas aeruginosa
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
-
727780
Burkholderia thailandensis
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-(methylthio)propanoate + CoA
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-methylmercaptopropionyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 14% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 15% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 18% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 19% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 4% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 5% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + 3-methylbutanoate + CoA
activity is 8% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + 3-methylbutanoyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 110% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 151% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 110% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acetate + CoA
activity is 151% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + acetyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 15% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 29% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 50% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 56% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 6% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 65% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 78% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 65% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 50% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 15% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 29% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + acrylate + CoA
activity is 6% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + acryloyl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 113% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 121% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 160% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 36% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 73% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 81% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 93% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 121% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 160% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 93% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 113% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + butyrate + CoA
activity is 81% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + butyryl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 109% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 2% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 32% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 41% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 63% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 63% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + crotonate + CoA
activity is 87% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + crotonyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 12% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 17% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 17% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 27% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 5% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 62% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + hexanoate + CoA
activity is 94% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + hexanoyl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 12% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 13% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 30% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 48% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 5% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + isobutyrate + CoA
activity is 7% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + isobutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 10% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 18% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 20% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 23% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 24% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 39% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + methylbutyrate + CoA
activity is 8% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + methylbutyryl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 179% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 18% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 31% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 49% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 51% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 68% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 91% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 68% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + pentanoate + CoA
activity is 179% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + pentanoyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 124% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 126% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 13% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 16% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 45% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 72% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 77% of the activity with 3-(methylthio)propanoate
727780
Ruegeria pomeroyi
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 124% of the activity with 3-(methylthio)propanoate
727780
Pseudomonas aeruginosa ATCC 15692
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 13% of the activity with 3-(methylthio)propanoate
727780
Candidatus Pelagibacter ubique HTCC1062
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 45% of the activity with 3-(methylthio)propanoate
727780
Burkholderia thailandensis ATCC 700388
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 72% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
ATP + propionate + CoA
activity is 126% of the activity with 3-(methylthio)propanoate
727780
Ruegeria lacuscaerulensis ITI-1157
AMP + diphosphate + propionyl-CoA
-
-
-
?
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Ruegeria pomeroyi
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Ruegeria lacuscaerulensis
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Burkholderia thailandensis
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Ruegeria pomeroyi
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Candidatus Pelagibacter ubique
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Ruegeria lacuscaerulensis
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Pseudomonas aeruginosa
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Pseudomonas aeruginosa ATCC 15692
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Candidatus Pelagibacter ubique HTCC1062
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Burkholderia thailandensis ATCC 700388
?
-
-
-
-
6.2.1.44
additional information
no activity with acetate, caprylate and caprate
727780
Ruegeria lacuscaerulensis ITI-1157
?
-
-
-
-
6.2.1.44
additional information
no activity with caprylate and caprate
727780
Ruegeria lacuscaerulensis ITI-1157
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.2.1.44
?
x * 64781, calculated from sequence
Burkholderia thailandensis
6.2.1.44
?
x * 59548, calculated from sequence
Pseudomonas aeruginosa
6.2.1.44
?
x * 59143, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
?
x * 59400, calculated from sequence
Ruegeria lacuscaerulensis
6.2.1.44
dimer
2 * 59000, SDS-PAGE; 2 * 61340, calculated from sequence
Candidatus Pelagibacter ubique
6.2.1.44
dimer
2 * 58000, SDS-PAGE; 2 * 59066, calculated from sequence
Ruegeria pomeroyi
6.2.1.44
dimer
2 * 59166, calculated from sequence; 2 * 62000, SDS-PAGE
Ruegeria pomeroyi
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.2.1.44
22
-
assay at room temperature
Candidatus Pelagibacter ubique
6.2.1.44
22
-
assay at room temperature
Ruegeria pomeroyi
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.2.1.44
10
30
activity at 10°C and at 30°C is nearly the same as at room temperature
Candidatus Pelagibacter ubique
6.2.1.44
10
30
activity at 10°C and at 30°C is nearly the same as at room temperature
Ruegeria pomeroyi
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.2.1.44
1
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
3.7
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
7.2
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
10.8
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
14.3
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
14.4
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
14.9
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
17.7
-
propionate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
18.7
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
22.3
-
Acrylate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
29.4
-
3-(methylthio)propanoate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
43.8
-
Butyrate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2.1.44
7
-
-
Ruegeria pomeroyi
6.2.1.44
7.5
-
-
Candidatus Pelagibacter ubique
6.2.1.44
7.5
-
-
Ruegeria pomeroyi
Expression
EC Number
Organism
Commentary
Expression
6.2.1.44
Ruegeria pomeroyi
two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on dimethylsulfoniopropionate and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation; two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on dimethylsulfoniopropionate and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation
additional information
General Information
EC Number
General Information
Commentary
Organism
6.2.1.44
metabolism
two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on DMSP and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation; two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on DMSP and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation
Ruegeria pomeroyi
General Information (protein specific)
EC Number
General Information
Commentary
Organism
6.2.1.44
metabolism
two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on DMSP and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation
Ruegeria pomeroyi
Expression (protein specific)
EC Number
Organism
Commentary
Expression
6.2.1.44
Ruegeria pomeroyi
two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on dimethylsulfoniopropionate and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation
additional information
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.2.1.44
0.2
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
1.2
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
16
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
50
-
Acrylate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
71
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
213
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
233
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
271
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
505
-
propionate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
735
-
3-(methylthio)propanoate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
1031
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
3370
-
Butyrate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.2.1.44
0.2
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
1.2
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
16
-
Acrylate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
50
-
Acrylate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
71
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
213
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
233
-
3-(methylthio)propanoate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
271
-
propionate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
505
-
propionate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
735
-
3-(methylthio)propanoate
pH 7.5, 22°C
Candidatus Pelagibacter ubique
6.2.1.44
1031
-
Butyrate
pH 7.5, 22°C
Ruegeria pomeroyi
6.2.1.44
3370
-
Butyrate
pH 7.5, 22°C
Candidatus Pelagibacter ubique