Literature summary extracted from
Szafran, M.; Skut, P.; Ditkowski, B.; Ginda, K.; Chandra, G.; Zakrzewska-Czerwinska, J.; Jakimowicz, D.
Topoisomerase I (TopA) is recruited to ParB complexes and is required for proper chromosome organization during Streptomyces coelicolor sporulation (2013), J. Bacteriol., 195, 4445-4455.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.6.2.1 |
gene topA, expression fo His-tagged enzyme under the control of a modified pET28 Plac promoter in Escherichia coli strain BL21 |
Streptomyces coelicolor |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.6.2.1 |
additional information |
TopA depletion in strains PS01 (wild-type background) and PS03 (-parB background). TopA depletion in the -parB background (PS03) leads to a decrease in chromosome segregation abnormalities with 8.9% anucleate prespores compared to that in the parB deletion strain J3305, with 19.5% anucleate prespores |
Streptomyces coelicolor |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
5.6.2.1 |
nucleus |
- |
Streptomyces coelicolor |
5634 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.6.2.1 |
Streptomyces coelicolor |
- |
topoisomerase I, TopA, is the only topoisomerase I homologue in the organism |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.6.2.1 |
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration |
Streptomyces coelicolor |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
5.6.2.1 |
hypha |
aerial |
Streptomyces coelicolor |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.6.2.1 |
additional information |
substrate is pUC19 plasmid DNA, and for supercoiling assay substrate pWHM3 plasmid DNA |
Streptomyces coelicolor |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.6.2.1 |
TopA |
- |
Streptomyces coelicolor |
5.6.2.1 |
Topoisomerase I |
- |
Streptomyces coelicolor |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.6.2.1 |
37 |
- |
assay at |
Streptomyces coelicolor |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.6.2.1 |
8.1 |
- |
assay at |
Streptomyces coelicolor |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
5.6.2.1 |
malfunction |
the enzyme depletion inhibits growth, blocks sporulation, and affects septation and chromosome condensation. But sporulation in the TopA-depleted strain can be partially restored by deletion of parB. Depletion of the TopA protein affects the secondary metabolism of Streptomyces coelicolor, leading to overproduction of actinorhodin |
Streptomyces coelicolor |
5.6.2.1 |
physiological function |
topoisomerase I is recruited to ParB complexes and is required for proper chromosome organization during Streptomyces coelicolor sporulation, overview |
Streptomyces coelicolor |