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Literature summary extracted from

  • Bourand, A.; Yebra, M.J.; Boel, G.; Maze, A.; Deutscher, J.
    Utilization of D-ribitol by Lactobacillus casei BL23 requires a mannose-type phosphotransferase system and three catabolic enzymes (2013), J. Bacteriol., 195, 2652-2661.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.1 DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.137 EDTA complete inhibition at 50 mM, the enzyme activity can be restored by adding 25 mM Zn2+ Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.137 additional information no increase in enzyme activity is observed when 25 mM Mg2+ or Mn2+ is added to the EDTA-containing assay mixture Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.137 D-ribitol 5-phosphate + NAD(P)+ Lacticaseibacillus casei
-
 D-ribulose 5-phosphate + NAD(P)H + H+
-
 ?
1.1.1.137 D-ribitol 5-phosphate + NAD(P)+ Lacticaseibacillus casei 64H
-
 D-ribulose 5-phosphate + NAD(P)H + H+
-
 ?
5.1.3.1 D-Ribulose 5-phosphate Lacticaseibacillus casei
-
 D-Xylulose 5-phosphate
-
 ?
5.1.3.1 D-Ribulose 5-phosphate Lacticaseibacillus casei
-
 D-Xylulose 5-phosphate
-
 r
5.1.3.1 D-Ribulose 5-phosphate Lacticaseibacillus casei 64H
-
 D-Xylulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.137 Lacticaseibacillus casei
-
-
-
5.1.3.1 Lacticaseibacillus casei
-
-
-
5.1.3.1 Lacticaseibacillus casei A0A510WIR9 gene rpe or LCABL_29200
-
5.1.3.1 Lacticaseibacillus casei 64H
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.1 recombinant His-tagged enzyme Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.137 76.8
-
 at pH 7.4, temperature not specified in the publication Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.137 D-ribitol 5-phosphate + NAD(P)+
-
 Lacticaseibacillus casei D-ribulose 5-phosphate + NAD(P)H + H+
-
 ?
1.1.1.137 D-ribitol 5-phosphate + NAD(P)+
-
 Lacticaseibacillus casei 64H D-ribulose 5-phosphate + NAD(P)H + H+
-
 ?
5.1.3.1 D-Ribulose 5-phosphate
-
 Lacticaseibacillus casei D-Xylulose 5-phosphate
-
 ?
5.1.3.1 D-Ribulose 5-phosphate
-
 Lacticaseibacillus casei D-Xylulose 5-phosphate
-
 r
5.1.3.1 D-Ribulose 5-phosphate
-
 Lacticaseibacillus casei 64H D-Xylulose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.137 D-Rtl-5-P DH
-
 Lacticaseibacillus casei
1.1.1.137 ribitol-5-P 2-dehydrogenase
-
 Lacticaseibacillus casei
1.1.1.137 rtpD
-
 Lacticaseibacillus casei
5.1.3.1 D-Ribulose-5-P 3-epimerase
-
 Lacticaseibacillus casei

General Information

EC Number General Information Comment Organism
5.1.3.1 metabolism D-ribulose-5-P 3-epimerase catalyzes the second step of D-ribitol catabolism. The enzyme is probably involved in an alternative ribitol degradation pathway, which might be functional in Lactobacillus casei strain 64H but not in strain BL23, because one of the BL23 genes carries a frameshift mutation Lacticaseibacillus casei