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Literature summary extracted from

  • Liu, L.; Zhang, L.; Tang, W.; Gu, Y.; Hua, Q.; Yang, S.; Jiang, W.; Yang, C.
    Phosphoketolase pathway for xylose catabolism in Clostridium acetobutylicum revealed by 13C metabolic flux analysis (2012), J. Bacteriol., 194, 5413-5422.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.9 Clostridium acetobutylicum Q97JE3
-
-
4.1.2.9 Clostridium acetobutylicum DSM 792 Q97JE3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.9 2.01
-
 pH 6.5, 37°C Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.9 D-Xylulose 5-phosphate + phosphate
-
 Clostridium acetobutylicum Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.1.2.9 D-Xylulose 5-phosphate + phosphate
-
 Clostridium acetobutylicum DSM 792 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.9 CAC1343
-
 Clostridium acetobutylicum

General Information

EC Number General Information Comment Organism
4.1.2.9 physiological function the phosphoketolase pathway is active and contributes up to 40% of the xylose catabolic flux in Clostridium acetobutylicum. The split ratio of the phosphoketolase pathway to the pentose phosphate pathway is markedly increased when the xylose concentration in the culture medium is increased from 10 to 20 g per liter. A phosphoketolase-overexpressing strain shows slightly increased rates of cell growth and xylose consumption during the exponential growth phase. During the subsequent solventogenic phase, the phosphoketolase-overexpressing strain exhibits a strongly reduced xylose uptake rate and solvent yields and a high level of accumulation of acetate up to 75 mM. Unlike the control strain, the phosphoketolase-overexpressing strain does not reassimilate acetate at the solventogenic phase Clostridium acetobutylicum