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Literature summary extracted from

  • Lagerbaeck, P.; Carlson, K.
    Amino acid residues in the GIY-YIG endonuclease II of phage T4 affecting sequence recognition and binding as well as catalysis (2008), J. Bacteriol., 190, 5533-5544.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.8 expression in Eschgerichia coli Tequatrovirus T4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.8 modeling based on structure of UvrC, PDB code 1YD0. Residues G49, R57, E118, and N130 in the putative catalytic surface are important for substrate recognition and binding. Residues G49 and R57 are essential for normal sequence recognition. and play a role in forming the DNA-binding surface and exposing the substrate scissile bond at the active site. Residues N130 and P127 likely contribute to positioning the catalytic domain correctly. Residue K76, part of a conserved NUMOD3 DNA-binding motif of homing endonucleases found to overlap the MR, affects both sequence recognition and catalysis Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.8 A83L similar to wild-type, cleavage activity is 20 to 70% of the nicking activity Tequatrovirus T4
3.1.21.8 E118A complete loss of cleavage activity Tequatrovirus T4
3.1.21.8 G49A complete loss of cleavage activity Tequatrovirus T4
3.1.21.8 I24A similar to wild-type, cleavage activity is 20 to 70% of the nicking activity Tequatrovirus T4
3.1.21.8 K12A similar to wild-type, cleavage activity is 20 to 70% of the nicking activity Tequatrovirus T4
3.1.21.8 K76A  much reduced catalytic activity Tequatrovirus T4
3.1.21.8 L16A similar to wild-type, cleavage activity is 20 to 70% of the nicking activity Tequatrovirus T4
3.1.21.8 L84P much reduced catalytic activity Tequatrovirus T4
3.1.21.8 N130A complete loss of cleavage activity Tequatrovirus T4
3.1.21.8 P127L much reduced catalytic activity Tequatrovirus T4
3.1.21.8 R57A cleavage activity is 5 to 7% of the nicking activity Tequatrovirus T4
3.1.21.8 S72A similar to wild-type, cleavage activity is 20 to 70% of the nicking activity Tequatrovirus T4
3.1.21.8 V90A similar to wild-type, cleavage activity is 20 to 70% of the nicking activity Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.8 Mg2+ required Tequatrovirus T4
3.1.21.8 Mn2+ compared to Mg2+, relative nicking activity is 0.4 with top strand, 0.8 with bottom strand Tequatrovirus T4
3.1.21.8 additional information ca2+ cannot substitute Mg2+ Tequatrovirus T4
3.1.21.8 Ni2+ compared to Mg2+, relative nicking activity is 0.1 with top strand, 0.1 with bottom strand Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.8 Tequatrovirus T4 P07059
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.21.8 denA
-
 Tequatrovirus T4