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Literature summary extracted from

  • Amo, T.; Atomi, H.; Imanaka, T.
    Biochemical properties and regulated gene expression of the superoxide dismutase from the facultatively aerobic hyperthermophile Pyrobaculum calidifontis (2003), J. Bacteriol., 185, 6340-6347.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 expressed in Escherichia coli. Although the recombinant protein is soluble, little activity is observed due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein Pyrobaculum calidifontis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 azide Mn2+-reconstituted recombinant enzyme and Fe2+-reconstututed recombinant enzyme displays relatively strong resistance against azide. Mn2+- and Fe2+-reconstituted activity decreases 50% with 380 and 340 mM azide, respectively Pyrobaculum calidifontis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 cytosol
-
 Pyrobaculum calidifontis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ native enzyme from aerobically-grown cells grown in standard medium contains 0.55 mol Fe2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with manganese contains less than 0.01 mol Fe2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with iron contains 0.01 mol Fe2+ per mol of subunit. Native enzyme from anaerobically-grown cells grown in standard medium contains 0.43 mol Fe2+ per mol of subunit. Recombinant apo-enzyme contains less than 0.01 mol Fe2+ per mol of subunit. Mn2+-reconstituted recombinant enzyme contains less than 0.01 mol Fe2+ per mol of subunit. Fe2+-reconstituted recombinant enzyme contains 0.76 mol Mn2+ per mol of subunit. The recombinant protein has little activity due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein Pyrobaculum calidifontis
1.15.1.1 Mn2+ native enzyme from aerobically-grown cells grown in standard medium contains 0.55 mol Mn2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with manganese contains 0.86 mol Mn2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with iron contains 0.68 mol Mn2+ per mol of subunit. Native enzyme from anaerobically-grown cells grown in standard medium contains 0.08 mol Mn2+ per mol of subunit. Recombinant apoenzyme contains less than 0.01 mol Mn2+ per m,ol of subunit. Mn2+-reconstituted recombinant enzyme contains 0.86 mol Mn2+ per mol of subunit. Fe2+-reconstituted recombinant enzyme contains less than 0.01 mol Mn2+ per mol of subunit.The recombinant protein has little activity due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein Pyrobaculum calidifontis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24000
-
 x * 24000, SDS-PAGE Pyrobaculum calidifontis
1.15.1.1 61000
-
 gel filtration Pyrobaculum calidifontis

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Pyrobaculum calidifontis Q76CY6
-
-
1.15.1.1 Pyrobaculum calidifontis JCM 11548 Q76CY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1
-
 Pyrobaculum calidifontis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 culture condition:aerobically-grown cell
-
 Pyrobaculum calidifontis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 434
-
 recombinant Fe2+-reconstituted enzyme, pH and temperature not specified in the publication Pyrobaculum calidifontis
1.15.1.1 1960
-
 purified native enzyme, pH and temperature not specified in the publication Pyrobaculum calidifontis
1.15.1.1 1970
-
 recombinant Mn2+-reconstituted enzyme, pH and temperature not specified in the publication Pyrobaculum calidifontis

Subunits

EC Number Subunits Comment Organism
1.15.1.1 ? x * 24000, SDS-PAGE Pyrobaculum calidifontis

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Pyrobaculum calidifontis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 100
-
 the Mn2+-reconstituted recombinant enzyme is not inactivated at all after 5 h of incubation at 100°C. A 1 h incubation leads to a 50% decrease in the activity of the Fe2+-reconstituted enzyme Pyrobaculum calidifontis