EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.B2 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.1.B2 | C124R | IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro | Escherichia coli |
4.3.1.B2 | E46G | IGP synthases formed with E46G mutant HisF subunit shows 2800fold reduction in the kcat/Km ratio for glutamine | Escherichia coli |
4.3.1.B2 | Q123R | IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro | Escherichia coli |
4.3.1.B2 | R5H | IGP synthases formed with R5H mutant HisF subunit shows 1500fold reduction in the kcat/Km ratio for glutamine | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.0125 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.0175 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.0218 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.0426 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 1 | 1.2 | NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 1 | 4.5 | L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 5.4 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 7.4 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 13.4 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 14.3 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.B2 | L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Escherichia coli | - |
L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.B2 | Escherichia coli | P60595 and P60664 | P60595: IGP synthase glutamine amidotransferase subunit, P60664: IGP synthase cyclase subunit | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.B2 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.B2 | L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Escherichia coli | L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
4.3.1.B2 | NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Escherichia coli | D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.1.B2 | heterodimer | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.B2 | 30 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.13 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.19 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 3.7 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 4.3 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 4.4 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 5.2 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 5.2 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 5.3 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 5.7 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 5.7 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.B2 | 8 | - |
assay at | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.013 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.024 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.392 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.399 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.425 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 0.581 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 120 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 240 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 300 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3.1.B2 | 300 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli |