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Literature summary extracted from

  • Klem, T.J.; Chen, Y.; Davisson, V.J.
    Subunit interactions and glutamine utilization by Escherichia coli imidazole glycerol phosphate synthase (2001), J. Bacteriol., 182, 989-996.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.B2
-
Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.B2 C124R IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro Escherichia coli
4.3.1.B2 E46G IGP synthases formed with E46G mutant HisF subunit shows 2800fold reduction in the kcat/Km ratio for glutamine Escherichia coli
4.3.1.B2 Q123R IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro Escherichia coli
4.3.1.B2 R5H IGP synthases formed with R5H mutant HisF subunit shows 1500fold reduction in the kcat/Km ratio for glutamine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.B2 0.0125
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 0.0175
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
4.3.1.B2 0.0218
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.3.1.B2 0.0426
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 1 1.2 NH4+ pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 1 4.5 L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 5.4
-
L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 7.4
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.3.1.B2 13.4
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
4.3.1.B2 14.3
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate Escherichia coli
-
L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.B2 Escherichia coli P60595 and P60664 P60595: IGP synthase glutamine amidotransferase subunit, P60664: IGP synthase cyclase subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.B2
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
Escherichia coli L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
4.3.1.B2 NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
Escherichia coli D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.B2 heterodimer
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.B2 30
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.B2 0.13
-
L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 0.19
-
L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 3.7
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 4.3
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.3.1.B2 4.4
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 5.2
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.3.1.B2 5.2
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 5.3
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
4.3.1.B2 5.7
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
4.3.1.B2 5.7
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.B2 8
-
assay at Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.B2 0.013
-
L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 0.024
-
L-glutamine pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 0.392
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 0.399
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 0.425
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli
4.3.1.B2 0.581
-
NH4+ pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.3.1.B2 120
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF Escherichia coli
4.3.1.B2 240
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF Escherichia coli
4.3.1.B2 300
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF Escherichia coli
4.3.1.B2 300
-
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF Escherichia coli