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Literature summary extracted from

  • Mai, B.; Frey, G.; Swanson, R.V.; Mathur, E.J.; Stetter, K.O.
    Molecular cloning and functional expression of a protein-serine/threonine phosphatase from the hyperthermophilic archaeon Pyrodictium abyssi TAG11 (1998), J. Bacteriol., 180, 4030-4035.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression in Escherichia coli Pyrodictium abyssi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 Cu2+
-
 Pyrodictium abyssi
3.1.3.16 diethyldicarbonate 0.5%. No inhibition at 0.05% and 0.1% Pyrodictium abyssi
3.1.3.16 EDTA
-
 Pyrodictium abyssi
3.1.3.16 Fe2+
-
 Pyrodictium abyssi
3.1.3.16 NaF
-
 Pyrodictium abyssi
3.1.3.16 okadaic acid inhibition is greater at 40°C than at 90°C Pyrodictium abyssi
3.1.3.16 sodium potassium tartrate
-
 Pyrodictium abyssi
3.1.3.16 Zn2+
-
 Pyrodictium abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Co2+ activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM Pyrodictium abyssi
3.1.3.16 Mg2+ activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation with Mg2+ is induced between 5 and 10 mM Pyrodictium abyssi
3.1.3.16 Mn2+ activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+ is the most effective divalent metal ion. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM Pyrodictium abyssi
3.1.3.16 Ni2+ activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM Pyrodictium abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 33340
-
 x * 33340, calculation from sequence Pyrodictium abyssi

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Pyrodictium abyssi O08367
-
-
3.1.3.16 Pyrodictium abyssi TAG11 O08367
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16
-
 Pyrodictium abyssi

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.16 the protein denatured by 6 M guanidinium chloride is refolded by dilution (100fold) into a buffer containing 0.5 M L-arginine Pyrodictium abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 [a peptide]-serine/threonine phosphate + H2O
-
 Pyrodictium abyssi [a peptide]-serine/threonine + phosphate
-
 ?
3.1.3.16 [a peptide]-serine/threonine phosphate + H2O
-
 Pyrodictium abyssi TAG11 [a peptide]-serine/threonine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 ? x * 33340, calculation from sequence Pyrodictium abyssi

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 protein-serine/threonine phosphatase
-
 Pyrodictium abyssi
3.1.3.16 Py-PP1
-
 Pyrodictium abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.16 90
-
-
 Pyrodictium abyssi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.16 40 110 the enzyme is active in the range from 40 to 110°C Pyrodictium abyssi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.16 90
-
 half-life: 180 min Pyrodictium abyssi
3.1.3.16 100
-
 half-life: 7 min Pyrodictium abyssi
3.1.3.16 105
-
 half-life: 3 min Pyrodictium abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 6
-
 assay at Pyrodictium abyssi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.16 4.5 8 pH changes (pH 4.5 to 8.0) have no significant effect on enzyme activity. No activity is observed below pH 4.0 or above 8.5 Pyrodictium abyssi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.16 Pyrodictium abyssi calculation from sequence
-
 6.15