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Literature summary extracted from

  • Jolley, K.A.; Rapaport, E.; Hough, D.W.; Danson, M.J.; Woods, W.G.; Dyall-Smith, M.L.
    Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: homologous overexpression of the cloned gene (1996), J. Bacteriol., 178, 3044-3048.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 overexpressed in the parent organism by using the halophilic archaeal rRNA promoter Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.018
-
 dihydrolipoamide pH 8.0, 37°C Haloferax volcanii
1.8.1.4 0.08
-
 NAD+ pH 8.0, 37°C Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.4 KCl maximal enzymatic activity at 2 to 3 M KCl Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 66500
-
 x * 66500, SDS-PAGE Haloferax volcanii

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Haloferax volcanii Q04829
-
-
1.8.1.4 Haloferax volcanii DSM 3757 Q04829
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4
-
 Haloferax volcanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 24
-
 pH 8.0, 37°C Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Haloferax volcanii lipoamide + NADH
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Haloferax volcanii DSM 3757 lipoamide + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 ? x * 66500, SDS-PAGE Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 9
-
-
 Haloferax volcanii