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Literature summary extracted from

  • Schauer, N.L.; Ferry, J.G.
    Composition of the coenzyme F420-dependent formate dehydrogenase from Methanobacterium formicicum (1986), J. Bacteriol., 165, 405-411.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.17.98.3 azide, FAD, glycerol, and 2-mercaptoethanol stabilize during purification Methanobacterium formicicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.98.3 Iron nonheme iron-sulfur flavoprotein, contains 20.24 iron Methanobacterium formicicum
1.17.98.3 Zn2+ contains 2 Zn2+ Methanobacterium formicicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.98.3 53000
-
 1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE Methanobacterium formicicum
1.17.98.3 85000
-
 1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE Methanobacterium formicicum
1.17.98.3 176000
-
 gel filtration Methanobacterium formicicum

Organism

EC Number Organism UniProt Comment Textmining
1.17.98.3 Methanobacterium formicicum
-
-
-
1.17.98.3 Methanobacterium formicicum DSM 2639
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.98.3
-
 Methanobacterium formicicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.98.3 36.2
-
 substrate: coenzyme F420, pH and temperature not specified in the publication Methanobacterium formicicum
1.17.98.3 447
-
 substrate: methyl viologen, pH and temperature not specified in the publication Methanobacterium formicicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.98.3 formate + oxidized coenzyme F420
-
 Methanobacterium formicicum CO2 + reduced coenzyme F420
-
 ?
1.17.98.3 formate + oxidized coenzyme F420
-
 Methanobacterium formicicum DSM 2639 CO2 + reduced coenzyme F420
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.98.3 dimer 1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE Methanobacterium formicicum

Synonyms

EC Number Synonyms Comment Organism
1.17.98.3 coenzyme F420-dependent formate dehydrogenase
-
 Methanobacterium formicicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.98.3 FAD nonheme iron-sulfur flavoprotein, contains 1 FAD molecule. Reduction of the enzyme facilitates dissociation of FAD, and the FAD-depleted enzyme is unable to reduce coenzyme F420. Preincubation of the FAD-depleted enzyme with FAD restores coenzyme F420-dependent activity Methanobacterium formicicum
1.17.98.3 molybdopterin denaturation of the enzyme releases a molybdopterin cofactor, the enzyme contains 1 mol of molybdenum Methanobacterium formicicum