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Literature summary extracted from
- Characterization and purification of carbon monoxide dehydrogenase from Methanosarcina barkeri (1984), J. Bacteriol., 158, 231-237.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.4 | KCN | 0.01 mM, 85% inhibition | Methanosarcina barkeri |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.4 | 5 | - |
CO | pH 7.0, 37°C | Methanosarcina barkeri | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.4 | Ni2+ | Ni is tightly bound to the enzyme and is not removed by anaerobic dialysis or gel permeation | Methanosarcina barkeri | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.4 | 18000 | - |
x * 92000 + x * 18000 | Methanosarcina barkeri |
| 1.2.7.4 | 92000 | - |
x * 92000 + x * 18000 | Methanosarcina barkeri |
| 1.2.7.4 | 232000 | - |
- |
Methanosarcina barkeri |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.7.4 | Methanosarcina barkeri | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.2.7.4 | Oxygen treatment results in an activity loss of 90% within 30 min. Treatment of oxygen-inactivated enzyme with CO, H2, or dithionite does not restore activity | Methanosarcina barkeri |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.7.4 | - |
Methanosarcina barkeri |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.2.7.4 | culture condition:acetate-grown cell | - |
Methanosarcina barkeri | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.4 | 216 | - |
pH 7.0, 37°C | Methanosarcina barkeri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.4 | CO + H2O + methyl viologen | - |
Methanosarcina barkeri | CO2 + reduced methyl viologen | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.7.4 | ? | x * 92000 + x * 18000 | Methanosarcina barkeri |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.7.4 | Carbon monoxide dehydrogenase | - |
Methanosarcina barkeri |
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