EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.98.3 | 1,10-phenanthroline | 5 mM, 33% inhibition | Methanobacterium formicicum | |
1.17.98.3 | 2,2'-dipyridyl | 5 mM, 87% inhibition | Methanobacterium formicicum | |
1.17.98.3 | azide | - |
Methanobacterium formicicum | |
1.17.98.3 | cyanide | - |
Methanobacterium formicicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.98.3 | 0.006 | - |
oxidized coenzyme F420 | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
1.17.98.3 | 0.013 | - |
FMN | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
1.17.98.3 | 0.025 | - |
FAD | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.98.3 | Molybdenum | the purified enzyme contains 0.0074 mM of molybdenum per g of protein | Methanobacterium formicicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.98.3 | 288000 | - |
gel filtration | Methanobacterium formicicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.98.3 | Methanobacterium formicicum | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.17.98.3 | the enzyme is extremely sensitive to oxygen. The exposure of crude cell-free extract to 101.3 kPa (1 atm) of air results in rapid loss of activity. The removal of air by vacuum degassing with O2-free N2 and the addition of 1 mM sodium dithionite does not restore activity.Sodium azide (10 mM) partially protected the against inactivation by air | Methanobacterium formicicum |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.98.3 | - |
Methanobacterium formicicum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.98.3 | 0.0009 | - |
substrate: FMN, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
1.17.98.3 | 0.0016 | - |
substrate: FAD, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
1.17.98.3 | 0.0082 | - |
substrate: oxidized coenzyme F420, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
1.17.98.3 | 0.059 | - |
substrate: oxidized methyl viologen, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.98.3 | formate + FAD | specific activity 5.4fold lower than for the reaction with coenzyme F420. Vmax /Km is 11fold as compared to reaction with coenzyme F420 | Methanobacterium formicicum | CO2 + FADH2 | - |
? | |
1.17.98.3 | formate + FMN | specific activity 9.1fold lower than for the reaction with coenzyme F420. Vmax /Km is 6.4fold as compared to reaction with coenzyme F420 | Methanobacterium formicicum | CO2 + FMNH2 | - |
? | |
1.17.98.3 | formate + oxidized coenzyme F420 | no activity with NAD+ or NADP* | Methanobacterium formicicum | CO2 + reduced coenzyme F420 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.98.3 | 55 | - |
- |
Methanobacterium formicicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.98.3 | 7.9 | - |
assayed with coenzyme F420 | Methanobacterium formicicum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.17.98.3 | 7.3 | 8.3 | pH 7.3: about 40% of maximal activity, pH 8.3: about 70% of maximal activity | Methanobacterium formicicum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.98.3 | 0.006 | - |
cyanide | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
1.17.98.3 | 0.039 | - |
azide | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |