EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.279 | expressed in Escherichia coli BL21 | Lachnoclostridium phytofermentans |
2.4.1.334 | expressed in Escherichia coli BL21 | Lachnoclostridium phytofermentans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.279 | 0.49 | - |
phosphate | pH 7.0, 30°C | Lachnoclostridium phytofermentans | |
2.4.1.279 | 1 | 7 | D-xylose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 1 | 7 | 1,5-anhydro-D-glucitol | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 1.2 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose | Lachnoclostridium phytofermentans | |
2.4.1.279 | 2.1 | - |
D-glucose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 20 | - |
D-galactose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 79 | - |
methyl alpha-D-glucopyranoside | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 1 | 2 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 2.6 | - |
phosphate | pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 2.9 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 3.1 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 3.2 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 3.8 | - |
maltose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 8.3 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose | Lachnoclostridium phytofermentans | |
2.4.1.334 | 11 | - |
phosphate | pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 50 | - |
kojibiose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.279 | cytosol | - |
Lachnoclostridium phytofermentans | 5829 | - |
2.4.1.334 | cytosol | - |
Lachnoclostridium phytofermentans | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.279 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | - |
D-glucose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.279 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans DSM 18823 | - |
D-glucose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans DSM 18823 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans DSM 18823 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.279 | Lachnoclostridium phytofermentans | A9KSL5 | - |
- |
2.4.1.279 | Lachnoclostridium phytofermentans DSM 18823 | A9KSL5 | - |
- |
2.4.1.334 | Lachnoclostridium phytofermentans | A9KSL6 | - |
- |
2.4.1.334 | Lachnoclostridium phytofermentans DSM 18823 | A9KSL6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.279 | - |
Lachnoclostridium phytofermentans |
2.4.1.334 | - |
Lachnoclostridium phytofermentans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.279 | 1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate | 26% activity compared to D-glucose as acceptor | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate | - |
r | |
2.4.1.279 | 1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate | 26% activity compared to D-glucose as acceptor | Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate | - |
r | |
2.4.1.279 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans | D-glucose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.279 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | D-glucose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.279 | D-galactose + beta-D-glucopyranose 1-phosphate | 21% activity compared to D-glucose as acceptor | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate | - |
r | |
2.4.1.279 | D-galactose + beta-D-glucopyranose 1-phosphate | 21% activity compared to D-glucose as acceptor | Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate | - |
r | |
2.4.1.279 | D-glucose + beta-D-glucopyranose 1-phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
2.4.1.279 | D-glucose + beta-D-glucopyranose 1-phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
2.4.1.279 | D-xylose + beta-D-glucopyranose 1-phosphate | 38% activity compared to D-glucose as acceptor | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate | - |
r | |
2.4.1.279 | methyl alpha-D-glucopyranoside + beta-D-glucopyranose 1-phosphate | 2.1% activity compared to D-glucose as acceptor | Lachnoclostridium phytofermentans | methyl 3-O-alpha-D-glucopyranosyl-D-glucopyranoside + phosphate | - |
r | |
2.4.1.279 | additional information | with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose | Lachnoclostridium phytofermentans | ? | - |
? | |
2.4.1.279 | additional information | with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose | Lachnoclostridium phytofermentans DSM 18823 | ? | - |
? | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | - |
r | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose | Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
ir | |
2.4.1.334 | 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate | 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose | Lachnoclostridium phytofermentans DSM 18823 | 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate | - |
ir | |
2.4.1.334 | kojibiose + beta-D-glucopyranose 1-phosphate | - |
Lachnoclostridium phytofermentans | ? + phosphate | - |
? | |
2.4.1.334 | maltose + beta-D-glucopyranose 1-phosphate | - |
Lachnoclostridium phytofermentans | ? + phosphate | - |
? | |
2.4.1.334 | additional information | the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose | Lachnoclostridium phytofermentans | ? | - |
? | |
2.4.1.334 | additional information | the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose | Lachnoclostridium phytofermentans DSM 18823 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.279 | Cphy_3313 | locus name | Lachnoclostridium phytofermentans |
2.4.1.334 | Cphy_3314 | locus name | Lachnoclostridium phytofermentans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.279 | 40 | - |
stable up to 40°C | Lachnoclostridium phytofermentans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.279 | 8.3 | - |
methyl alpha-D-glucopyranoside | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 25 | - |
D-galactose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 30 | - |
1,5-anhydro-D-glucitol | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 31 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C | Lachnoclostridium phytofermentans | |
2.4.1.279 | 40 | - |
D-xylose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 52 | - |
D-glucose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 58 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose | Lachnoclostridium phytofermentans | |
2.4.1.334 | 2.7 | - |
maltose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 3.5 | - |
kojibiose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 5.1 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose | Lachnoclostridium phytofermentans | |
2.4.1.334 | 8.5 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 8.7 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 11 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 17 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.279 | 7 | - |
- |
Lachnoclostridium phytofermentans |
2.4.1.334 | 6.5 | - |
synthetic acitvity | Lachnoclostridium phytofermentans |
2.4.1.334 | 8 | - |
phosphorolytic activity | Lachnoclostridium phytofermentans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.279 | 5.5 | 9 | - |
Lachnoclostridium phytofermentans |
2.4.1.334 | 5.5 | 9.5 | - |
Lachnoclostridium phytofermentans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.279 | 0.11 | - |
methyl alpha-D-glucopyranoside | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 1.3 | - |
D-galactose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 1.8 | - |
1,5-anhydro-D-glucitol | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 2.4 | - |
D-xylose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 24 | - |
D-glucose | pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.279 | 49 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose | Lachnoclostridium phytofermentans | |
2.4.1.334 | 0.069 | - |
kojibiose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 0.5 | - |
maltose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 1.4 | - |
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans | |
2.4.1.334 | 1.6 | - |
beta-D-glucopyranose 1-phosphate | pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose | Lachnoclostridium phytofermentans | |
2.4.1.334 | 2.8 | - |
3-O-alpha-D-glucopyranosyl-D-glucopyranose | pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate | Lachnoclostridium phytofermentans |