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Literature summary extracted from

  • Nihira, T.; Nishimoto, M.; Nakai, H.; Ohtsubo, K.; Kitaoka, M.
    Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans (2014), J. Appl. Glycosci., 61, 59-66.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.279 expressed in Escherichia coli BL21 Lachnoclostridium phytofermentans
2.4.1.334 expressed in Escherichia coli BL21 Lachnoclostridium phytofermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.279 0.49
-
phosphate pH 7.0, 30°C Lachnoclostridium phytofermentans
2.4.1.279 1 7 D-xylose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 1 7 1,5-anhydro-D-glucitol pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 1.2
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose Lachnoclostridium phytofermentans
2.4.1.279 2.1
-
D-glucose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 20
-
D-galactose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 79
-
methyl alpha-D-glucopyranoside pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 1 2 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 2.6
-
phosphate pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 2.9
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 3.1
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 3.2
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 3.8
-
maltose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 8.3
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose Lachnoclostridium phytofermentans
2.4.1.334 11
-
phosphate pH 7.0, 30°C, phosphorolysis reaction of 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 50
-
kojibiose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.279 cytosol
-
Lachnoclostridium phytofermentans 5829
-
2.4.1.334 cytosol
-
Lachnoclostridium phytofermentans 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans
-
D-glucose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans DSM 18823
-
D-glucose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans DSM 18823
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans DSM 18823
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.279 Lachnoclostridium phytofermentans A9KSL5
-
-
2.4.1.279 Lachnoclostridium phytofermentans DSM 18823 A9KSL5
-
-
2.4.1.334 Lachnoclostridium phytofermentans A9KSL6
-
-
2.4.1.334 Lachnoclostridium phytofermentans DSM 18823 A9KSL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.279
-
Lachnoclostridium phytofermentans
2.4.1.334
-
Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.279 1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate 26% activity compared to D-glucose as acceptor Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
-
r
2.4.1.279 1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate 26% activity compared to D-glucose as acceptor Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
-
r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans D-glucose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans DSM 18823 D-glucose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.279 D-galactose + beta-D-glucopyranose 1-phosphate 21% activity compared to D-glucose as acceptor Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
r
2.4.1.279 D-galactose + beta-D-glucopyranose 1-phosphate 21% activity compared to D-glucose as acceptor Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
r
2.4.1.279 D-glucose + beta-D-glucopyranose 1-phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
r
2.4.1.279 D-glucose + beta-D-glucopyranose 1-phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
r
2.4.1.279 D-xylose + beta-D-glucopyranose 1-phosphate 38% activity compared to D-glucose as acceptor Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
-
r
2.4.1.279 methyl alpha-D-glucopyranoside + beta-D-glucopyranose 1-phosphate 2.1% activity compared to D-glucose as acceptor Lachnoclostridium phytofermentans methyl 3-O-alpha-D-glucopyranosyl-D-glucopyranoside + phosphate
-
r
2.4.1.279 additional information with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose Lachnoclostridium phytofermentans ?
-
?
2.4.1.279 additional information with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose Lachnoclostridium phytofermentans DSM 18823 ?
-
?
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
-
r
2.4.1.334 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
ir
2.4.1.334 3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate 3-O-alpha-D-glucopyranosyl-D-glucopyranose i.e. nigerose Lachnoclostridium phytofermentans DSM 18823 3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
ir
2.4.1.334 kojibiose + beta-D-glucopyranose 1-phosphate
-
Lachnoclostridium phytofermentans ? + phosphate
-
?
2.4.1.334 maltose + beta-D-glucopyranose 1-phosphate
-
Lachnoclostridium phytofermentans ? + phosphate
-
?
2.4.1.334 additional information the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose Lachnoclostridium phytofermentans ?
-
?
2.4.1.334 additional information the enzyme does not significantly phosphorylyze trehalose, kojibiose, nigerose, and maltose Lachnoclostridium phytofermentans DSM 18823 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.279 Cphy_3313 locus name Lachnoclostridium phytofermentans
2.4.1.334 Cphy_3314 locus name Lachnoclostridium phytofermentans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.279 40
-
stable up to 40°C Lachnoclostridium phytofermentans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.279 8.3
-
methyl alpha-D-glucopyranoside pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 25
-
D-galactose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 30
-
1,5-anhydro-D-glucitol pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 31
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C Lachnoclostridium phytofermentans
2.4.1.279 40
-
D-xylose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 52
-
D-glucose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 58
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose Lachnoclostridium phytofermentans
2.4.1.334 2.7
-
maltose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 3.5
-
kojibiose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 5.1
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose Lachnoclostridium phytofermentans
2.4.1.334 8.5
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 8.7
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 11
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 17
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.279 7
-
-
Lachnoclostridium phytofermentans
2.4.1.334 6.5
-
synthetic acitvity Lachnoclostridium phytofermentans
2.4.1.334 8
-
phosphorolytic activity Lachnoclostridium phytofermentans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.279 5.5 9
-
Lachnoclostridium phytofermentans
2.4.1.334 5.5 9.5
-
Lachnoclostridium phytofermentans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.279 0.11
-
methyl alpha-D-glucopyranoside pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 1.3
-
D-galactose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 1.8
-
1,5-anhydro-D-glucitol pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 2.4
-
D-xylose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 24
-
D-glucose pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 49
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose Lachnoclostridium phytofermentans
2.4.1.334 0.069
-
kojibiose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 0.5
-
maltose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 1.4
-
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.334 1.6
-
beta-D-glucopyranose 1-phosphate pH 7.0, 30°C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose Lachnoclostridium phytofermentans
2.4.1.334 2.8
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate Lachnoclostridium phytofermentans