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Literature summary extracted from

  • Oberthuer, M.; Leimkuhler, C.; Kruger, R.G.; Lu, W.; Walsh, C.T.; Kahne, D.
    A systematic investigation of the synthetic utility of glycopeptide glycosyltransferases (2005), J. Am. Chem. Soc., 127, 10747-10752.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.322 0.038
-
 dTDP-beta-L-daunosamine pH not specified in the publication, temperature not specified in the publication Amycolatopsis orientalis
2.4.1.322 0.232
-
 dTDP-beta-L-acosamine pH not specified in the publication, temperature not specified in the publication Amycolatopsis orientalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.322 Amycolatopsis orientalis Q9AFC7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.322 dTDP-beta-L-acosamine + devancosaminyl-vancomycin
-
 Amycolatopsis orientalis dTDP + ?
-
 ?
2.4.1.322 dTDP-beta-L-daunosamine + devancosaminyl-vancomycin
-
 Amycolatopsis orientalis dTDP + ?
-
 ?
2.4.1.322 dTDP-beta-L-vancosamine + devancosaminyl-vancomycin natural substrate Amycolatopsis orientalis dTDP + vancomycin
-
 ?
2.4.1.322 additional information enzyme shows moderately relaxed donor substrate specificities for the glycosylation of its natural aglycone. It transfers 4-epi-L-vancosamine, L-daunosamine, and L-acosamine with good turnover. 2-Deoxy-L-glucose, 2-deoxy-L-rhamnose, and L-ristosamine are transferred in trace amounts, whereas for substrates containing an amino group at C-4, no product is observed Amycolatopsis orientalis ?
-
 ?
2.4.1.322 UDP-4-epi-L-vancosamine + devancosaminyl-vancomycin
-
 Amycolatopsis orientalis UDP + epi-L-vancomycin epivancomycin is a hybrid teicoplanin/vancomycin glycopeptide ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.322 GtfD
-
 Amycolatopsis orientalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.322 2.13
-
 dTDP-beta-L-daunosamine pH not specified in the publication, temperature not specified in the publication Amycolatopsis orientalis
2.4.1.322 2.25
-
 dTDP-beta-L-acosamine pH not specified in the publication, temperature not specified in the publication Amycolatopsis orientalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.322 9.7
-
 dTDP-beta-L-acosamine pH not specified in the publication, temperature not specified in the publication Amycolatopsis orientalis
2.4.1.322 56.7
-
 dTDP-beta-L-daunosamine pH not specified in the publication, temperature not specified in the publication Amycolatopsis orientalis