EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.14 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Caldicellulosiruptor saccharolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 1.03 | - |
L-rhamnose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 3 | 3.5 | D-ribose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 3.64 | - |
L-Mannose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 3.65 | - |
L-Lyxose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 14.3 | - |
D-allose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.14 | Co2+ | maximum activity in the presence of Co2+ | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | Mn2+ | the enzyme shows 58% of the maximum activity in the presence of Mn2+ | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | additional information | no activity in the presence of Na+, Mg2+, Ca2+, Cu2+, K+, and Zn2+ or in the absence of any ions | Caldicellulosiruptor saccharolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 48000 | - |
4 * 48000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
5.3.1.14 | 48292 | - |
4 * 48292, MALDI-TOF mass spectrometry | Caldicellulosiruptor saccharolyticus |
5.3.1.14 | 48294 | - |
4 * 48294, calculated from amino acid sequence | Caldicellulosiruptor saccharolyticus |
5.3.1.14 | 193400 | - |
gel filtration | Caldicellulosiruptor saccharolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.14 | L-Rhamnose | Caldicellulosiruptor saccharolyticus | - |
L-Rhamnulose | - |
r | |
5.3.1.14 | L-Rhamnose | Caldicellulosiruptor saccharolyticus ATCC 43494 | - |
L-Rhamnulose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.14 | Caldicellulosiruptor saccharolyticus | A4XHV7 | - |
- |
5.3.1.14 | Caldicellulosiruptor saccharolyticus ATCC 43494 | A4XHV7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.14 | heat treatment, nucleic acid precipitation, and Q-Sepharose ion-exchange chromatography | Caldicellulosiruptor saccharolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 14.8 | - |
crude extract, in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 90°C | Caldicellulosiruptor saccharolyticus |
5.3.1.14 | 379 | - |
after 25.6fold purification, in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 90°C | Caldicellulosiruptor saccharolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.14 | D-allose | - |
Caldicellulosiruptor saccharolyticus | D-psicose | - |
? | |
5.3.1.14 | D-allose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | D-psicose | - |
? | |
5.3.1.14 | D-ribose | very minimal activity toward D-ribose | Caldicellulosiruptor saccharolyticus | D-ribulose | - |
? | |
5.3.1.14 | D-ribose | very minimal activity toward D-ribose | Caldicellulosiruptor saccharolyticus ATCC 43494 | D-ribulose | - |
? | |
5.3.1.14 | L-lyxose | - |
Caldicellulosiruptor saccharolyticus | L-xylulose | - |
? | |
5.3.1.14 | L-lyxose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | L-xylulose | - |
? | |
5.3.1.14 | L-Mannose | - |
Caldicellulosiruptor saccharolyticus | L-Fructose | - |
? | |
5.3.1.14 | L-Mannose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | L-Fructose | - |
? | |
5.3.1.14 | L-Rhamnose | - |
Caldicellulosiruptor saccharolyticus | L-Rhamnulose | - |
r | |
5.3.1.14 | L-Rhamnose | - |
Caldicellulosiruptor saccharolyticus ATCC 43494 | L-Rhamnulose | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.14 | homotetramer | 4 * 48000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
5.3.1.14 | homotetramer | 4 * 48292, MALDI-TOF mass spectrometry | Caldicellulosiruptor saccharolyticus |
5.3.1.14 | homotetramer | 4 * 48294, calculated from amino acid sequence | Caldicellulosiruptor saccharolyticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.14 | L-RhI | - |
Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 90 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 80 | 90 | the enzyme shows good thermostability and retains more than 90% activity after a 6 h incubation at 80°C and pH 7.0-8.0. The enzyme is stable at 85°C for 4 h. However, the enzyme activity drops sharply to 30% after 2 h at 90°C, and the half-life at 90°C is around 65 min | Caldicellulosiruptor saccharolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 68.1 | - |
D-allose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 78.7 | - |
L-Mannose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 99.9 | - |
L-rhamnose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 118 | - |
D-ribose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 186 | - |
L-Lyxose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 7 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 4 | 11 | the enzyme is stable at pH values ranging from 4.0 to 11.0 with more than 80% remaining activity and retains more than 90% activity after a 6 h incubation at 80°C and pH 7.0-8.0. The enzyme activity is fully retained at pH 7-8, 32% remaining at pH 9.0, but becomes inactivated in the pH range from 4.0 to 6.0 | Caldicellulosiruptor saccharolyticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 3.54 | - |
D-ribose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 4.77 | - |
D-allose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 21.6 | - |
L-Mannose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 50.8 | - |
L-Lyxose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus | |
5.3.1.14 | 97.1 | - |
L-rhamnose | in 50 mM potassium phosphate buffer (pH 7.0) and 1 mM Co2+, at 65°C | Caldicellulosiruptor saccharolyticus |