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Literature summary extracted from

  • Liu, S.Y.; Aliyari, R.; Chikere, K.; Li, G.; Marsden, M.D.; Smith, J.K.; Pernet, O.; Guo, H.; Nusbaum, R.; Zack, J.A.; Freiberg, A.N.; Su, L.; Lee, B.; Cheng, G.
    Interferon-inducible cholesterol-25-hydroxylase broadly inhibits viral entry by production of 25-hydroxycholesterol (2013), Immunity, 38, 92-105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.38 gene CH25H, overexpression in HEK-293T cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.38 cholesterol + AH2 + O2 Mus musculus
-
 25-hydroxycholesterol + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.38 Mus musculus Q9Z0F5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.38 bone marrow-derived macrophage
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.38 cholesterol + AH2 + O2
-
 Mus musculus 25-hydroxycholesterol + A + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.38 CH25H
-
 Mus musculus
1.14.99.38 cholesterol-25-hydroxylase
-
 Mus musculus

Expression

EC Number Organism Comment Expression
1.14.99.38 Mus musculus both IFN-alpha- and IFN-gamma induce the enzyme in bone marrow-derived macrophages up

General Information

EC Number General Information Comment Organism
1.14.99.38 malfunction loss of function of Ch25h leads to susceptibility to viral infections in vitro Mus musculus
1.14.99.38 physiological function cholesterol-25-hydroxylase acts as a broadly antiviral interferon-stimulated gene. CH25H converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol. 25-Hydroxycholesterol treatment in cultured cells broadly inhibits growth of enveloped viruses including VSV, HSV, HIV, and MHV68 and acutely pathogenic EBOV, RVFV, RSSEV, and Nipah viruses. The compound inhibits cell entry of viruses HIV and VSV, and inhibits viral protein-mediated cell-cell membrane fusion of virus NIV. 25-Hydroxycholesterol directly modifies membranes Mus musculus