EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.16 | glycerol | the recombinant enzyme CPS1 is activated by glycerol in the absence of N-acetyl-L-glutamate | Homo sapiens | |
6.3.4.16 | N-acetyl-L-glutamate | essential activator | Homo sapiens | |
6.3.4.16 | N-carbamyl-L-glutamate | - |
Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.16 | expressed in Escherichia coli DH10Bac cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.16 | A1378T | the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity | Homo sapiens |
6.3.4.16 | A438P | inactive | Homo sapiens |
6.3.4.16 | Al38P | the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity | Homo sapiens |
6.3.4.16 | G1376S | the mutation has no detectable effect on activity | Homo sapiens |
6.3.4.16 | L1381S | the mutation is clearly disease-causing (carbamoyl-phosphate synthetase deficiency), since it induces strong enzyme instability | Homo sapiens |
6.3.4.16 | L390R | the mutation is clearly disease-causing (carbamoyl-phosphate synthetase deficiency), since it induces strong enzyme instability | Homo sapiens |
6.3.4.16 | N355D | the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity | Homo sapiens |
6.3.4.16 | T1443A | the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity | Homo sapiens |
6.3.4.16 | T344A | the mutation has no detectable effect on activity | Homo sapiens |
6.3.4.16 | T544M | the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity | Homo sapiens |
6.3.4.16 | Y389C | the carbamoyl-phosphate synthetase deficiency-causing mutation greatly decreases enzyme activity | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.16 | glycerol | in the presence of N-acetyl-L-glutamate the enzyme is inhibited by increasing concentrations of glycerol | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.16 | 0.43 | - |
ATP | mutant enzyme Y389C, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.45 | - |
ATP | mutant enzyme G1376S, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.45 | - |
ATP | mutant enzyme T344A, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.47 | - |
ATP | recombinant wild type enzyme, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.57 | - |
ATP | mutant enzyme N355D, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.62 | - |
ATP | mutant enzyme T1443A, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.9 | - |
ATP | mutant enzyme A1378T, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 0.9 | - |
NH4+ | mutant enzyme A1378T, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1 | - |
NH4+ | mutant enzyme N355D, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1 | - |
NH4+ | mutant enzyme T1443A, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1 | - |
NH4+ | recombinant wild type enzyme, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1.1 | - |
NH4+ | mutant enzyme G1376S, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1.1 | - |
NH4+ | mutant enzyme Y389C, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1.2 | - |
NH4+ | mutant enzyme T344A, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1.3 | - |
NH4+ | mutant enzyme T544M, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 1.92 | - |
ATP | mutant enzyme T544M, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 3.6 | - |
HCO3- | mutant enzyme T1443A, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 4 | - |
HCO3- | recombinant wild type enzyme, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 4.2 | - |
HCO3- | mutant enzyme Y389C, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 4.3 | - |
HCO3- | mutant enzyme A1378T, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 4.3 | - |
HCO3- | mutant enzyme T344A, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 4.5 | - |
HCO3- | mutant enzyme G1376S, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 4.6 | - |
HCO3- | mutant enzyme N355D, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens | |
6.3.4.16 | 242 | - |
HCO3- | mutant enzyme T544M, in 50 mM glycyl-glycine pH 7.4, 70 mM KCl, 1 mM dithiothreitol, 20 mM MgSO4, at 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.4.16 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.16 | 192000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
6.3.4.16 | 202000 | - |
native enzyme, gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.16 | 2 ATP + NH4+ + HCO3- | Homo sapiens | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.16 | Homo sapiens | P31327 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.16 | HisTrap column chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.4.16 | intestine | - |
Homo sapiens | - |
6.3.4.16 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.16 | 2 ATP + NH4+ + HCO3- | - |
Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.16 | carbamoyl-phosphate synthetase 1 | - |
Homo sapiens |
6.3.4.16 | CPS1 | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.16 | ATP | - |
Homo sapiens |